The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology

Overview

Journal Genomics Proteomics Bioinformatics

Specialty Biology

Date 2013 Feb 19

PMID 23414612

Citations 18

Authors

Fei Chen

Mengxing Dong

Meng Ge

Lingxiang Zhu

Lufeng Ren

Guocheng Liu

Rong Mu

Affiliations

Soon will be listed here.

Abstract

DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the "REAP" method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application.

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