Modulation of Lipopolysaccharide-induced Chorioamnionitis by Ureaplasma Parvum in Sheep

Overview

Journal Am J Obstet Gynecol

Publisher Elsevier

Specialty Gynecology & Obstetrics

Date 2013 Feb 16

PMID 23410690

Citations 15

Authors

Candice C Snyder

Katherine B Wolfe

Tate Gisslen

Christine L Knox

Matthew W Kemp

Boris W Kramer

John P Newnham

Alan H Jobe

Suhas G Kallapur

Affiliations

Soon will be listed here.

Abstract

Objective: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS).

Study Design: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 ± 1 day of gestation.

Results: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion.

Conclusion: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.

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