Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts

Overview

Journal Avicenna J Med Biotechnol

Specialty Biomedical Engineering

Date 2013 Feb 15

PMID 23408761

Citations 4

Authors

Babak Qasemi-Panahi

Parviz Tajik

Mansoureh Movahedin

Gholamali Moghaddam

Younes Barzgar

Hamed Heidari-Vala

Affiliations

Soon will be listed here.

Abstract

Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show their multipotentiality. In the present study, bovine SSCs were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were identified by Vimentin and Oct-4 immunocytochemical staining method, respectively. In order to differentiate SSCs into osteoblasts, we used consecutive inducer media without separation of the colonies. We characterized osteoblasts using Alizarin red staining.

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