Robust Polarity Establishment Occurs Via an Endocytosis-based Cortical Corralling Mechanism

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2013 Feb 13

PMID 23401000

Citations 46

Authors

Mini Jose

Sylvain Tollis

Deepak Nair

Jean-Baptiste Sibarita

Derek McCusker

Affiliations

Soon will be listed here.

Abstract

Formation of a stable polarity axis underlies numerous biological processes. Here, using high-resolution imaging and complementary mathematical modeling we find that cell polarity can be established via the spatial coordination of opposing membrane trafficking activities: endocytosis and exocytosis. During polarity establishment in budding yeast, these antagonistic processes become apposed. Endocytic vesicles corral a central exocytic zone, tightening it to a vertex that establishes the polarity axis for the ensuing cell cycle. Concomitantly, the endocytic system reaches an equilibrium where internalization events occur at a constant frequency. Endocytic mutants that failed to initiate periodic internalization events within the corral displayed wide, unstable polarity axes. These results, predicted by in silico modeling and verified by high resolution in vivo studies, identify a requirement for endocytic corralling during robust polarity establishment.

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