Cross-sectional Study Reveals High Prevalence of Clostridium Difficile Non-PCR Ribotype 078 Strains in Australian Veal Calves at Slaughter

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2013 Feb 12

PMID 23396338

Citations 43

Authors

Daniel R Knight

Sara Thean

Papanin Putsathit

Stan Fenwick

Thomas V Riley

Affiliations

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Abstract

Recent reports in North America and Europe of Clostridium difficile being isolated from livestock and retail meats of bovine origin have raised concerns about the risk to public health. To assess the situation in Australia, we investigated the prevalence and genetic diversity of C. difficile in adult cattle and calves at slaughter. Carcass washings, gastrointestinal contents, and feces were collected from abattoirs across five Australian states. Selective culture, toxin profiling, and PCR ribotyping were performed. The prevalence of C. difficile was 56% (203/360 samples) in feces from <7-day-old calves, 3.8% (1/26) in 2- to 6-month-old calves, and 1.8% (5/280) in adult cattle. Three PCR ribotypes (RTs), RT127, RT033, and RT126, predominated in <7-day-old calves and comprised 77.8% (158/203 samples) of isolates. RT056, which has not been reported in cattle before, was found in 16 <7-day-old calves (7.7%). Surprisingly, RT078 strains, which dominate production animal carriage studies in the Northern Hemisphere, were not isolated.

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References

Thean S, Elliott B, Riley T . Clostridium difficile in horses in Australia--a preliminary study. J Med Microbiol. 2011; 60(Pt 8):1188-1192. DOI: 10.1099/jmm.0.030908-0. View

Costa M, Stampfli H, Arroyo L, Pearl D, Weese J . Epidemiology of Clostridium difficile on a veal farm: prevalence, molecular characterization and tetracycline resistance. Vet Microbiol. 2011; 152(3-4):379-84. DOI: 10.1016/j.vetmic.2011.05.014. View

Bakker D, Corver J, Harmanus C, Goorhuis A, Keessen E, Fawley W . Relatedness of human and animal Clostridium difficile PCR ribotype 078 isolates determined on the basis of multilocus variable-number tandem-repeat analysis and tetracycline resistance. J Clin Microbiol. 2010; 48(10):3744-9. PMC: 2953124. DOI: 10.1128/JCM.01171-10. View

Koene M, Mevius D, Wagenaar J, Harmanus C, Hensgens M, Meetsma A . Clostridium difficile in Dutch animals: their presence, characteristics and similarities with human isolates. Clin Microbiol Infect. 2011; 18(8):778-84. DOI: 10.1111/j.1469-0691.2011.03651.x. View

Rodriguez-Palacios A, Reid-Smith R, Staempfli H, Daignault D, Janecko N, Avery B . Possible seasonality of Clostridium difficile in retail meat, Canada. Emerg Infect Dis. 2009; 15(5):802-5. PMC: 2687045. DOI: 10.3201/eid1505.081084. View

Barbut F, Decre D, Lalande V, Burghoffer B, Noussair L, Gigandon A . Clinical features of Clostridium difficile-associated diarrhoea due to binary toxin (actin-specific ADP-ribosyltransferase)-producing strains. J Med Microbiol. 2005; 54(Pt 2):181-185. DOI: 10.1099/jmm.0.45804-0. View

Zidaric V, Pardon B, Dos Vultos T, Deprez P, Brouwer M, Roberts A . Different antibiotic resistance and sporulation properties within multiclonal Clostridium difficile PCR ribotypes 078, 126, and 033 in a single calf farm. Appl Environ Microbiol. 2012; 78(24):8515-22. PMC: 3502897. DOI: 10.1128/AEM.02185-12. View

Kuntz J, Chrischilles E, Pendergast J, Herwaldt L, Polgreen P . Incidence of and risk factors for community-associated Clostridium difficile infection: a nested case-control study. BMC Infect Dis. 2011; 11:194. PMC: 3154181. DOI: 10.1186/1471-2334-11-194. View

Kato H, Kato N, Watanabe K, Iwai N, Nakamura H, Yamamoto T . Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR. J Clin Microbiol. 1998; 36(8):2178-82. PMC: 105000. DOI: 10.1128/JCM.36.8.2178-2182.1998. View

10.

Braun V, Hundsberger T, Leukel P, Sauerborn M, von Eichel-Streiber C . Definition of the single integration site of the pathogenicity locus in Clostridium difficile. Gene. 1996; 181(1-2):29-38. DOI: 10.1016/s0378-1119(96)00398-8. View

11.

Hensgens M, Keessen E, Squire M, Riley T, Koene M, de Boer E . Clostridium difficile infection in the community: a zoonotic disease?. Clin Microbiol Infect. 2012; 18(7):635-45. DOI: 10.1111/j.1469-0691.2012.03853.x. View

12.

Wiegand P, Nathwani D, Wilcox M, Stephens J, Shelbaya A, Haider S . Clinical and economic burden of Clostridium difficile infection in Europe: a systematic review of healthcare-facility-acquired infection. J Hosp Infect. 2012; 81(1):1-14. DOI: 10.1016/j.jhin.2012.02.004. View

13.

Keessen E, Gaastra W, Lipman L . Clostridium difficile infection in humans and animals, differences and similarities. Vet Microbiol. 2011; 153(3-4):205-17. DOI: 10.1016/j.vetmic.2011.03.020. View

14.

He M, Sebaihia M, Lawley T, Stabler R, Dawson L, Martin M . Evolutionary dynamics of Clostridium difficile over short and long time scales. Proc Natl Acad Sci U S A. 2010; 107(16):7527-32. PMC: 2867753. DOI: 10.1073/pnas.0914322107. View

15.

Dingle K, Griffiths D, Didelot X, Evans J, Vaughan A, Kachrimanidou M . Clinical Clostridium difficile: clonality and pathogenicity locus diversity. PLoS One. 2011; 6(5):e19993. PMC: 3098275. DOI: 10.1371/journal.pone.0019993. View

16.

Hammitt M, Bueschel D, Keel M, Glock R, Cuneo P, DeYoung D . A possible role for Clostridium difficile in the etiology of calf enteritis. Vet Microbiol. 2007; 127(3-4):343-52. PMC: 7131641. DOI: 10.1016/j.vetmic.2007.09.002. View

17.

Riley T . Is Clostridium difficile a threat to Australia's biosecurity?. Med J Aust. 2009; 190(12):661-2. DOI: 10.5694/j.1326-5377.2009.tb02630.x. View

18.

Kato N, Ou C, Kato H, Bartley S, Brown V, Dowell Jr V . Identification of toxigenic Clostridium difficile by the polymerase chain reaction. J Clin Microbiol. 1991; 29(1):33-7. PMC: 269697. DOI: 10.1128/jcm.29.1.33-37.1991. View

19.

Rodriguez-Palacios A, Stampfli H, Duffield T, Peregrine A, Trotz-Williams L, Arroyo L . Clostridium difficile PCR ribotypes in calves, Canada. Emerg Infect Dis. 2007; 12(11):1730-6. PMC: 3372327. DOI: 10.3201/eid1211.051581. View

20.

Keel K, Brazier J, Post K, Weese S, Songer J . Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J Clin Microbiol. 2007; 45(6):1963-4. PMC: 1933037. DOI: 10.1128/JCM.00224-07. View