Protein Determinants of Meiotic DNA Break Hot Spots

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2013 Feb 12

PMID 23395004

Citations 39

Authors

Kyle R Fowler

Susana Gutierrez-Velasco

Cristina Martin-Castellanos

Gerald R Smith

Affiliations

Soon will be listed here.

Abstract

Meiotic recombination, crucial for proper chromosome segregation and genome evolution, is initiated by programmed DNA double-strand breaks (DSBs) in yeasts and likely all sexually reproducing species. In fission yeast, DSBs occur up to hundreds of times more frequently at special sites, called hot spots, than in other regions of the genome. What distinguishes hot spots from cold regions is an unsolved problem, although transcription factors determine some hot spots. We report the discovery that three coiled-coil proteins-Rec25, Rec27, and Mug20-bind essentially all hot spots with great specificity even without DSB formation. These small proteins are components of linear elements, are related to synaptonemal complex proteins, and are essential for nearly all DSBs at most hot spots. Our results indicate these hot spot determinants activate or stabilize the DSB-forming protein Rec12 (Spo11 homolog) rather than promote its binding to hot spots. We propose a paradigm for hot spot determination and crossover control by linear element proteins.

Citing Articles

Mug20-Rec25-Rec27 binds DNA and enhances meiotic DNA break formation via phase-separated condensates.

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Chromosome architecture and homologous recombination in meiosis.

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