Improvised Microinjection Technique for Mosquito Vectors

Overview

Journal Indian J Med Res

Specialty General Medicine

Date 2013 Feb 9

PMID 23391792

Citations 6

Authors

S Sampath Kumar

H P Puttaraju

Affiliations

Soon will be listed here.

Abstract

Background & Objectives: Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection.

Methods: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult.

Results: The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability.

Interpretation & Conclusions: The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.

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