Single-cell Photonic Nanocavity Probes

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2013 Feb 8

PMID 23387382

Citations 12

Authors

Gary Shambat

Sri-Rajasekhar Kothapalli

J Provine

Tomas Sarmiento

James Harris

Sanjiv Sam Gambhir

Jelena Vuckovic

Affiliations

Soon will be listed here.

Abstract

In this report, we demonstrate for the first time photonic nanocavities operating inside single biological cells. Here we develop a nanobeam photonic crystal (PC) cavity as an advanced cellular nanoprobe, active in nature, and configurable to provide a multitude of actions for both intracellular sensing and control. Our semiconductor nanocavity probes emit photoluminescence (PL) from embedded quantum dots (QD) and sustain high quality resonant photonic modes inside cells. The probes are shown to be minimally cytotoxic to cells from viability studies, and the beams can be loaded in cells and tracked for days at a time, with cells undergoing regular division with the beams. We present in vitro label-free protein sensing with our probes to detect streptavidin as a path towards real-time biomarker and biomolecule detection inside single cells. The results of this work will enable new areas of research merging the strengths of photonic nanocavities with fundamental cell biology.

Citing Articles

Monitoring Various Bioactivities at the Molecular, Cellular, Tissue, and Organism Levels via Biological Lasers.

Shan H, Dai H, Chen X Sensors (Basel). 2022; 22(9).

PMID: 35590841 PMC: 9102053. DOI: 10.3390/s22093149.

Review of biosensing with whispering-gallery mode lasers.

Toropov N, Cabello G, Serrano M, Gutha R, Rafti M, Vollmer F Light Sci Appl. 2021; 10(1):42.

PMID: 33637696 PMC: 7910454. DOI: 10.1038/s41377-021-00471-3.

Single-cell biomagnifier for optical nanoscopes and nanotweezers.

Li Y, Liu X, Li B Light Sci Appl. 2019; 8:61.

PMID: 31645911 PMC: 6804537. DOI: 10.1038/s41377-019-0168-4.

Applications of Photonic Crystal Nanobeam Cavities for Sensing.

Qiao Q, Xia J, Lee C, Zhou G Micromachines (Basel). 2019; 9(11).

PMID: 30715040 PMC: 6267459. DOI: 10.3390/mi9110541.

Non-obstructive intracellular nanolasers.

Fikouras A, Schubert M, Karl M, Kumar J, Powis S, Di Falco A Nat Commun. 2018; 9(1):4817.

PMID: 30446665 PMC: 6240115. DOI: 10.1038/s41467-018-07248-0.

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