In-gel Determination of L-amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-forming Reaction

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Feb 6

PMID 23383337

Citations 6

Authors

Zhiliang Yu

Ning Zhou

Chuntian Zhao

Juanping Qiu

Affiliations

Soon will be listed here.

Abstract

L-amino acid oxidase (LAAO) is attracting increasing attention due to its important functions. Diverse detection methods with their own properties have been developed for characterization of LAAO. In the present study, a simple, rapid, sensitive, cost-effective and reproducible method for quantitative in-gel determination of LAAO activity based on the visualization of Prussian blue-forming reaction is described. Coupled with SDS-PAGE, this Prussian blue agar assay can be directly used to determine the numbers and approximate molecular weights of LAAO in one step, allowing straightforward application for purification and sequence identification of LAAO from diverse samples.

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References

Yang H, Johnson P, Ko K, Kamio M, Germann M, Derby C . Cloning, characterization and expression of escapin, a broadly antimicrobial FAD-containing L-amino acid oxidase from ink of the sea hare Aplysia californica. J Exp Biol. 2005; 208(Pt 18):3609-22. DOI: 10.1242/jeb.01795. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Lucas-Elio P, Gomez D, Solano F, Sanchez-Amat A . The antimicrobial activity of marinocine, synthesized by Marinomonas mediterranea, is due to hydrogen peroxide generated by its lysine oxidase activity. J Bacteriol. 2006; 188(7):2493-501. PMC: 1428416. DOI: 10.1128/JB.188.7.2493-2501.2006. View

Ehara T, Kitajima S, Kanzawa N, Tamiya T, Tsuchiya T . Antimicrobial action of achacin is mediated by L-amino acid oxidase activity. FEBS Lett. 2002; 531(3):509-12. DOI: 10.1016/s0014-5793(02)03608-6. View

Roth M . Fluorescence reaction for amino acids. Anal Chem. 1971; 43(7):880-2. DOI: 10.1021/ac60302a020. View

Okubo B, Silva O, Migliolo L, Gomes D, Porto W, Batista C . Evaluation of an antimicrobial L-amino acid oxidase and peptide derivatives from Bothropoides mattogrosensis pitviper venom. PLoS One. 2012; 7(3):e33639. PMC: 3306279. DOI: 10.1371/journal.pone.0033639. View

Saito M, Seki M, Iida K, Nakayama H, Yoshida S . A novel agar medium to detect hydrogen peroxide-producing bacteria based on the prussian blue-forming reaction. Microbiol Immunol. 2007; 51(9):889-92. DOI: 10.1111/j.1348-0421.2007.tb03971.x. View

Vallon O, Bulte L, Kuras R, Olive J, Wollman F . Extensive accumulation of an extracellular L-amino-acid oxidase during gametogenesis of Chlamydomonas reinhardtii. Eur J Biochem. 1993; 215(2):351-60. DOI: 10.1111/j.1432-1033.1993.tb18041.x. View

Sakurai Y, Takatsuka H, Yoshioka A, Matsui T, Suzuki M, Titani K . Inhibition of human platelet aggregation by L-amino acid oxidase purified from Naja naja kaouthia venom. Toxicon. 2001; 39(12):1827-33. DOI: 10.1016/s0041-0101(01)00133-7. View

10.

Gomez D, Espinosa E, Bertazzo M, Lucas-Elio P, Solano F, Sanchez-Amat A . The macromolecule with antimicrobial activity synthesized by Pseudoalteromonas luteoviolacea strains is an L-amino acid oxidase. Appl Microbiol Biotechnol. 2008; 79(6):925-30. DOI: 10.1007/s00253-008-1499-x. View

11.

Huang Y, Li M, Yu Z, Qian P . Correlation between pigmentation and larval settlement deterrence by Pseudoalteromonas sp. sf57. Biofouling. 2011; 27(3):287-93. DOI: 10.1080/08927014.2011.562978. View

12.

Singh S, Gogoi B, Bezbaruah R . Optimization of medium and cultivation conditions for L-amino acid oxidase production by Aspergillus fumigatus. Can J Microbiol. 2009; 55(9):1096-102. DOI: 10.1139/w09-068. View

13.

Yu Z, Qiao H . Advances in non-snake venom L-amino acid oxidase. Appl Biochem Biotechnol. 2012; 167(1):1-13. DOI: 10.1007/s12010-012-9611-1. View

14.

Davis M, Askin M, Hynes M . Amino acid catabolism by an areA-regulated gene encoding an L-amino acid oxidase with broad substrate specificity in Aspergillus nidulans. Appl Environ Microbiol. 2005; 71(7):3551-5. PMC: 1168990. DOI: 10.1128/AEM.71.7.3551-3555.2005. View

15.

Kitani Y, Mori T, Nagai H, Toyooka K, Ishizaki S, Shimakura K . Gene expression and distribution of antibacterial L-amino acid oxidase in the rockfish Sebastes schlegeli. Fish Shellfish Immunol. 2007; 23(6):1178-86. DOI: 10.1016/j.fsi.2007.04.005. View

16.

Rau J, Fischer U . In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production. J Microbiol Methods. 2011; 85(3):228-9. DOI: 10.1016/j.mimet.2011.03.002. View

17.

Ralph E, Anderson M, Cleland W, Fitzpatrick P . Mechanistic studies of the flavoenzyme tryptophan 2-monooxygenase: deuterium and 15N kinetic isotope effects on alanine oxidation by an L-amino acid oxidase. Biochemistry. 2006; 45(51):15844-52. PMC: 1994567. DOI: 10.1021/bi061894o. View