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In Vivo Detection of Hemoglobin Oxygen Saturation and Carboxyhemoglobin Saturation with Multiwavelength Photoacoustic Microscopy

Overview
Journal Opt Lett
Specialty Ophthalmology
Date 2013 Feb 6
PMID 23381275
Citations 24
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Abstract

A method for noninvasively detecting hemoglobin oxygen saturation (SO2) and carboxyhemoglobin saturation (SCO) in subcutaneous microvasculature with multiwavelength photoacoustic microscopy is presented. Blood samples mixed with different concentrations of carboxyhemoglobin were used to test the feasibility and accuracy of photoacoustic microscopy compared with the blood-gas analyzer. Moreover, fixed-point detection of SO2 and SCO in mouse ear was obtained, and the changes from normoxia to carbon monoxide hypoxia were dynamically monitored in vivo. Experimental results demonstrate that multiwavelength photoacoustic microscopy can detect SO2 and SCO, which has future potential clinical applications.

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