Survival, Sublethal Injury, and Recovery of Environmental Burkholderia Pseudomallei in Soil Subjected to Desiccation

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2013 Feb 5

PMID 23377947

Citations 6

Authors

Eloise Larsen

James J Smith

Robert Norton

Maree Corkeron

Affiliations

Soon will be listed here.

Abstract

Environmental Burkholderia pseudomallei isolated from sandy soil at Castle Hill, Townsville, in the dry tropic region of Queensland, Australia, was inoculated into sterile-soil laboratory microcosms subjected to variable soil moisture. Survival and sublethal injury of the B. pseudomallei strain were monitored by recovery using culture-based methods. Soil extraction buffer yielded higher recoveries as an extraction agent than sterile distilled water. B. pseudomallei was not recoverable when inoculated into desiccated soil but remained recoverable from moist soil subjected to 91 days' desiccation and showed a growth response to increased soil moisture over at least 113 days. Results indicate that endemic dry tropic soil may act as a reservoir during the dry season, with an increase in cell number and potential for mobilization from soil into water in the wet season.

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