A New Model for Preclinical Testing of Dermal Substitutes for Human Skin Reconstruction

Overview

Journal Pediatr Surg Int

Specialties General Surgery
Pediatrics

Date 2013 Feb 2

PMID 23371301

Citations 6

Authors

Fabienne Hartmann-Fritsch

Thomas Biedermann

Erik Braziulis

Martin Meuli

Ernst Reichmann

Affiliations

Soon will be listed here.

Abstract

Background: Currently, acellular dermal substitutes used for skin reconstruction are usually covered with split-thickness skin grafts. The goal of this study was to develop an animal model in which such dermal substitutes can be tested under standardized conditions using a bioengineered dermo-epidermal skin graft for coverage.

Methods: Bioengineered grafts consisting of collagen type I hydrogels with incorporated human fibroblasts and human keratinocytes seeded on these gels were produced. Two different dermal substitutes, namely Matriderm(®), and an acellular collagen type I hydrogel, were applied onto full-thickness skin wounds created on the back of immuno-incompetent rats. As control, no dermal substitute was used. As coverage for the dermal substitutes either the bioengineered grafts were used, or, as controls, human split-thickness skin or neonatal rat epidermis were used. Grafts were excised 21 days post-transplantation. Histology and immunofluorescence was performed to investigate survival, epidermis formation, and vascularization of the grafts.

Results: The bioengineered grafts survived on all tested dermal substitutes. Epidermis formation and vascularization were comparable to the controls.

Conclusion: We could successfully use human bioengineered grafts to test different dermal substitutes. This novel model can be used to investigate newly designed dermal substitutes in detail and in a standardized way.

Citing Articles

Acellular dermal matrix in skin wound healing in rabbits - histological and histomorphometric analyses.

Carvalho-Junior J, Zanata F, Aloise A, Ferreira L Clinics (Sao Paulo). 2021; 76:e2066.

PMID: 33681941 PMC: 7920408. DOI: 10.6061/clinics/2021/e2066.

Development of humanized mouse and rat models with full-thickness human skin and autologous immune cells.

Agarwal Y, Beatty C, Ho S, Thurlow L, Das A, Kelly S Sci Rep. 2020; 10(1):14598.

PMID: 32884084 PMC: 7471691. DOI: 10.1038/s41598-020-71548-z.

Down-Regulation of MHC Class I Expression in Human Keratinocytes Using Viral Vectors Containing Gene of Human Cytomegalovirus and Cultivation on Bovine Collagen-Elastin Matrix (Matriderm): Potential Approach for an Immune-Privileged Skin Substitute.

Schlottmann F, Strauss S, Hake K, Vogt P, Bucan V Int J Mol Sci. 2019; 20(9).

PMID: 31027326 PMC: 6540026. DOI: 10.3390/ijms20092056.

Impact of human mesenchymal cells of different body site origins on the maturation of dermo-epidermal skin substitutes.

Michalak-Micka K, Klar A, Bottcher-Haberzeth S, Reichmann E, Meuli M, Biedermann T Pediatr Surg Int. 2018; 35(1):121-127.

PMID: 30382375 DOI: 10.1007/s00383-018-4383-5.

Engineering epithelial-stromal interactions in vitro for toxicology assessment.

Belair D, Abbott B Toxicology. 2017; 382:93-107.

PMID: 28285100 PMC: 5985517. DOI: 10.1016/j.tox.2017.03.007.

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