Direct Assessment of Metabolite Utilization by Pseudomonas Aeruginosa During Growth on Artificial Sputum Medium

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2013 Jan 29

PMID 23354718

Citations 13

Authors

Volker Behrends

Benedikt Geier

Huw D Williams

Jacob G Bundy

Affiliations

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Abstract

We grew Pseudomonas aeruginosa in LB and artificial sputum medium (ASM) (filtered and unfiltered) and quantified metabolite utilization and excretion by nuclear magnetic resonance (NMR) spectroscopy (metabolic footprinting or extracellular metabolomics). Utilization rates were similar between media, but there were differences in excretion-e.g., acetate was produced only in unfiltered ASM.

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