Fabrication and Characterization of Multiscale Electrospun Scaffolds for Cartilage Regeneration

Overview

Journal Biomed Mater

Specialty Biomedical Engineering

Date 2013 Jan 29

PMID 23353096

Citations 21

Authors

Erica J Levorson

Perumcherry Raman Sreerekha

Krishna Prasad Chennazhi

F Kurtis Kasper

Shantikumar V Nair

Antonios G Mikos

Affiliations

Soon will be listed here.

Abstract

Recently, scaffolds for tissue regeneration purposes have been observed to utilize nanoscale features in an effort to reap the cellular benefits of scaffold features resembling extracellular matrix (ECM) components. However, one complication surrounding electrospun nanofibers is limited cellular infiltration. One method to ameliorate this negative effect is by incorporating nanofibers into microfibrous scaffolds. This study shows that it is feasible to fabricate electrospun scaffolds containing two differently scaled fibers interspersed evenly throughout the entire construct as well as scaffolds containing fibers composed of two discrete materials, specifically fibrin and poly(ε-caprolactone). In order to accomplish this, multiscale fibrous scaffolds of different compositions were generated using a dual extrusion electrospinning setup with a rotating mandrel. These scaffolds were then characterized for fiber diameter, porosity and pore size and seeded with human mesenchymal stem cells to assess the influence of scaffold architecture and composition on cellular responses as determined by cellularity, histology and glycosaminoglycan (GAG) content. Analysis revealed that nanofibers within a microfiber mesh function to maintain scaffold cellularity under serum-free conditions as well as aid the deposition of GAGs. This supports the hypothesis that scaffolds with constituents more closely resembling native ECM components may be beneficial for cartilage regeneration.

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References

Ramis J, Rubert M, Vondrasek J, Gaya A, Lyngstadaas S, Monjo M . Effect of enamel matrix derivative and of proline-rich synthetic peptides on the differentiation of human mesenchymal stem cells toward the osteogenic lineage. Tissue Eng Part A. 2012; 18(11-12):1253-63. DOI: 10.1089/ten.tea.2011.0404. View

Geng X, Kwon O, Jang J . Electrospinning of chitosan dissolved in concentrated acetic acid solution. Biomaterials. 2005; 26(27):5427-32. DOI: 10.1016/j.biomaterials.2005.01.066. View

Bolis S, Handley C, Comper W . Passive loss of proteoglycan from articular cartilage explants. Biochim Biophys Acta. 1989; 993(2-3):157-67. DOI: 10.1016/0304-4165(89)90158-x. View

Li W, Laurencin C, Caterson E, Tuan R, Ko F . Electrospun nanofibrous structure: a novel scaffold for tissue engineering. J Biomed Mater Res. 2002; 60(4):613-21. DOI: 10.1002/jbm.10167. View

Liao J, Guo X, Grande-Allen K, Kasper F, Mikos A . Bioactive polymer/extracellular matrix scaffolds fabricated with a flow perfusion bioreactor for cartilage tissue engineering. Biomaterials. 2010; 31(34):8911-20. PMC: 2953640. DOI: 10.1016/j.biomaterials.2010.07.110. View

Blomback B, Blomback M, EDMAN P, HESSEL B . Human fibrinopeptides. Isolation, characterization and structure. Biochim Biophys Acta. 1966; 115(2):371-96. DOI: 10.1016/0304-4165(66)90437-5. View

Lowery J, Datta N, Rutledge G . Effect of fiber diameter, pore size and seeding method on growth of human dermal fibroblasts in electrospun poly(epsilon-caprolactone) fibrous mats. Biomaterials. 2009; 31(3):491-504. DOI: 10.1016/j.biomaterials.2009.09.072. View

Capelli C, Gotti E, Morigi M, Rota C, Weng L, Dazzi F . Minimally manipulated whole human umbilical cord is a rich source of clinical-grade human mesenchymal stromal cells expanded in human platelet lysate. Cytotherapy. 2011; 13(7):786-801. DOI: 10.3109/14653249.2011.563294. View

Kern S, Eichler H, Stoeve J, Kluter H, Bieback K . Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-301. DOI: 10.1634/stemcells.2005-0342. View

10.

Pham Q, Sharma U, Mikos A . Electrospun poly(epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules. 2006; 7(10):2796-805. DOI: 10.1021/bm060680j. View

11.

Nitya G, Nair G, Mony U, Chennazhi K, Nair S . In vitro evaluation of electrospun PCL/nanoclay composite scaffold for bone tissue engineering. J Mater Sci Mater Med. 2012; 23(7):1749-61. DOI: 10.1007/s10856-012-4647-x. View

12.

Duggal S, Fronsdal K, Szoke K, Shahdadfar A, Melvik J, Brinchmann J . Phenotype and gene expression of human mesenchymal stem cells in alginate scaffolds. Tissue Eng Part A. 2009; 15(7):1763-73. DOI: 10.1089/ten.tea.2008.0306. View

13.

Mahmood T, Shastri V, van Blitterswijk C, Langer R, Riesle J . Tissue engineering of bovine articular cartilage within porous poly(ether ester) copolymer scaffolds with different structures. Tissue Eng. 2005; 11(7-8):1244-53. DOI: 10.1089/ten.2005.11.1244. View

14.

Soliman S, Pagliari S, Rinaldi A, Forte G, Fiaccavento R, Pagliari F . Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning. Acta Biomater. 2009; 6(4):1227-37. DOI: 10.1016/j.actbio.2009.10.051. View

15.

Seeberger K, Dufour J, Shapiro A, Lakey J, Rajotte R, Korbutt G . Expansion of mesenchymal stem cells from human pancreatic ductal epithelium. Lab Invest. 2006; 86(2):141-53. DOI: 10.1038/labinvest.3700377. View

16.

Chen W, Yao C, Wei Y, Chu I . Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold. Cytotechnology. 2010; 63(1):13-23. PMC: 3021150. DOI: 10.1007/s10616-010-9314-9. View

17.

Kawasaki K, Ochi M, Uchio Y, Adachi N, Matsusaki M . Hyaluronic acid enhances proliferation and chondroitin sulfate synthesis in cultured chondrocytes embedded in collagen gels. J Cell Physiol. 1999; 179(2):142-8. DOI: 10.1002/(SICI)1097-4652(199905)179:2<142::AID-JCP4>3.0.CO;2-Q. View

18.

Medrado G, Machado C, Valerio P, Sanches M, Goes A . The effect of a chitosan-gelatin matrix and dexamethasone on the behavior of rabbit mesenchymal stem cells. Biomed Mater. 2008; 1(3):155-61. DOI: 10.1088/1748-6041/1/3/010. View

19.

Alvarez-Barreto J, Linehan S, Shambaugh R, Sikavitsas V . Flow perfusion improves seeding of tissue engineering scaffolds with different architectures. Ann Biomed Eng. 2007; 35(3):429-42. DOI: 10.1007/s10439-006-9244-z. View

20.

Li W, Tuli R, Huang X, Laquerriere P, Tuan R . Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials. 2005; 26(25):5158-66. DOI: 10.1016/j.biomaterials.2005.01.002. View