In Vitro Modeling of Human Bocavirus 1 Infection of Polarized Primary Human Airway Epithelia

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2013 Jan 25

PMID 23345515

Citations 39

Authors

Xuefeng Deng

Ziying Yan

Yong Luo

Jian Xu

Fang Cheng

Yi Li

John F Engelhardt

Jianming Qiu

Affiliations

Soon will be listed here.

Abstract

Human bocavirus 1 (HBoV1) is an emerging human-pathogenic respiratory virus. We characterized two important features of HBoV1 infection in polarized primary human airway epithelia (HAE). Apical HBoV1 infection of HAE at a low multiplicity of infection causes disruption of the tight junction barrier, loss of cilia, and epithelial cell hypertrophy, which are hallmarks of the airway epithelial damage caused by HBoV1 infection. HBoV1 also infects HAE from the basolateral surface productively, although less efficiently, and this also leads to the characteristic airway epithelial damage.

Citing Articles

Advanced approaches to overcome biological barriers in respiratory and systemic routes of administration for enhanced nucleic acid delivery to the lung.

Kwak G, Lee D, Suk J Expert Opin Drug Deliv. 2023; 20(11):1531-1552.

PMID: 37946533 PMC: 10872418. DOI: 10.1080/17425247.2023.2282535.

Breaking Bad: Inflammasome Activation by Respiratory Viruses.

Cerato J, da Silva E, Porto B Biology (Basel). 2023; 12(7).

PMID: 37508374 PMC: 10376673. DOI: 10.3390/biology12070943.

Gene therapy for cystic fibrosis: Challenges and prospects.

Sui H, Xu X, Su Y, Gong Z, Yao M, Liu X Front Pharmacol. 2022; 13:1015926.

PMID: 36304167 PMC: 9592762. DOI: 10.3389/fphar.2022.1015926.

The small nonstructural protein NP1 of human bocavirus 1 directly interacts with Ku70 and RPA70 and facilitates viral DNA replication.

Ning K, Wang Z, Cheng F, Yan Z, Qiu J PLoS Pathog. 2022; 18(6):e1010578.

PMID: 35653410 PMC: 9197078. DOI: 10.1371/journal.ppat.1010578.

The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia.

Shao L, Ning K, Wang J, Cheng F, Wang S, Qiu J J Virol. 2021; 96(4):e0184021.

PMID: 34878919 PMC: 8865542. DOI: 10.1128/JVI.01840-21.

References

Allander T, Jartti T, Gupta S, Niesters H, Lehtinen P, Osterback R . Human bocavirus and acute wheezing in children. Clin Infect Dis. 2007; 44(7):904-10. PMC: 7107819. DOI: 10.1086/512196. View

Zhang L, Collins P, Lamb R, Pickles R . Comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (W3A), parainfluenza virus type 3, and respiratory syncytial virus. Virology. 2011; 421(1):67-77. PMC: 3208758. DOI: 10.1016/j.virol.2011.08.020. View

Dijkman R, Koekkoek S, Molenkamp R, Schildgen O, van der Hoek L . Human bocavirus can be cultured in differentiated human airway epithelial cells. J Virol. 2009; 83(15):7739-48. PMC: 2708629. DOI: 10.1128/JVI.00614-09. View

Villenave R, Thavagnanam S, Sarlang S, Parker J, Douglas I, Skibinski G . In vitro modeling of respiratory syncytial virus infection of pediatric bronchial epithelium, the primary target of infection in vivo. Proc Natl Acad Sci U S A. 2012; 109(13):5040-5. PMC: 3323997. DOI: 10.1073/pnas.1110203109. View

Pyrc K, Sims A, Dijkman R, Jebbink M, Long C, Deming D . Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures. J Virol. 2010; 84(21):11255-63. PMC: 2953148. DOI: 10.1128/JVI.00947-10. View

Hao W, Bernard K, Patel N, Ulbrandt N, Feng H, Svabek C . Infection and propagation of human rhinovirus C in human airway epithelial cells. J Virol. 2012; 86(24):13524-32. PMC: 3503113. DOI: 10.1128/JVI.02094-12. View

Soderlund-Venermo M, Lahtinen A, Jartti T, Hedman L, Kemppainen K, Lehtinen P . Clinical assessment and improved diagnosis of bocavirus-induced wheezing in children, Finland. Emerg Infect Dis. 2009; 15(9):1423-30. PMC: 2819894. DOI: 10.3201/eid1509.090204. View

Wang G, Deering C, Macke M, Shao J, Burns R, Blau D . Human coronavirus 229E infects polarized airway epithelia from the apical surface. J Virol. 2000; 74(19):9234-9. PMC: 102122. DOI: 10.1128/jvi.74.19.9234-9239.2000. View

Karp P, Moninger T, Weber S, Nesselhauf T, Launspach J, Zabner J . An in vitro model of differentiated human airway epithelia. Methods for establishing primary cultures. Methods Mol Biol. 2002; 188:115-37. DOI: 10.1385/1-59259-185-X:115. View

10.

Sims A, Baric R, Yount B, Burkett S, Collins P, Pickles R . Severe acute respiratory syndrome coronavirus infection of human ciliated airway epithelia: role of ciliated cells in viral spread in the conducting airways of the lungs. J Virol. 2005; 79(24):15511-24. PMC: 1316022. DOI: 10.1128/JVI.79.24.15511-15524.2005. View

11.

McClure C, Cole K, Wulff P, Klugmann M, Murray A . Production and titering of recombinant adeno-associated viral vectors. J Vis Exp. 2011; (57):e3348. PMC: 3308604. DOI: 10.3791/3348. View

12.

Allander T, Tammi M, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B . Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A. 2005; 102(36):12891-6. PMC: 1200281. DOI: 10.1073/pnas.0504666102. View

13.

Wang G, Slepushkin V, Bodner M, Zabner J, van Es H, Thomas P . Keratinocyte growth factor induced epithelial proliferation facilitates retroviral-mediated gene transfer to distal lung epithelia in vivo. J Gene Med. 2000; 1(1):22-30. DOI: 10.1002/(sici)1521-2254(199901/02)1:1<22::aid-jgm1>3.3.co;2-o. View

14.

Don M, Soderlund-Venermo M, Valent F, Lahtinen A, Hedman L, Canciani M . Serologically verified human bocavirus pneumonia in children. Pediatr Pulmonol. 2009; 45(2):120-6. DOI: 10.1002/ppul.21151. View

15.

Zabner J, Zeiher B, Friedman E, Welsh M . Adenovirus-mediated gene transfer to ciliated airway epithelia requires prolonged incubation time. J Virol. 1996; 70(10):6994-7003. PMC: 190750. DOI: 10.1128/JVI.70.10.6994-7003.1996. View

16.

Zhang L, Peeples M, Boucher R, Collins P, Pickles R . Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology. J Virol. 2002; 76(11):5654-66. PMC: 137037. DOI: 10.1128/jvi.76.11.5654-5666.2002. View

17.

Huang Q, Deng X, Yan Z, Cheng F, Luo Y, Shen W . Establishment of a reverse genetics system for studying human bocavirus in human airway epithelia. PLoS Pathog. 2012; 8(8):e1002899. PMC: 3431310. DOI: 10.1371/journal.ppat.1002899. View

18.

Ursic T, Steyer A, Kopriva S, Kalan G, Krivec U, Petrovec M . Human bocavirus as the cause of a life-threatening infection. J Clin Microbiol. 2011; 49(3):1179-81. PMC: 3067724. DOI: 10.1128/JCM.02362-10. View

19.

Ayers M, Jeffery P . Proliferation and differentiation in mammalian airway epithelium. Eur Respir J. 1988; 1(1):58-80. View

20.

Nascimento-Carvalho C, Cardoso M, Meriluoto M, Kemppainen K, Kantola K, Ruuskanen O . Human bocavirus infection diagnosed serologically among children admitted to hospital with community-acquired pneumonia in a tropical region. J Med Virol. 2011; 84(2):253-8. DOI: 10.1002/jmv.22268. View