Ability to Cause Erythema Migrans Differs Between Borrelia Burgdorferi Sensu Lato Isolates

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2013 Jan 24

PMID 23339549

Citations 16

Authors

Ellen Tijsse-Klasen

Nenad Pandak

Paul Hengeveld

Katsuhisa Takumi

Marion P G Koopmans

Hein Sprong

Affiliations

Soon will be listed here.

Abstract

Background: Lyme borreliosis is a tick-borne disease caused by Borrelia burgdorferi sensu lato. The variety of characteristic and non-specific clinical manifestations is partially explained by its genetic diversity. We investigated the ability of B. burgdorferi sl isolates to cause erythema migrans.

Methods: The genetic constellation of isolates from ticks was compared to isolates found in erythema migrans. PCR and sequence analysis was performed on the plasmid-encoded ospC and the chromosomal 5S-23S rDNA spacer region (IGS).

Results: Seven different B. burgdorferi sl genospecies were identified in 152 borrelia isolates from ticks and erythema migrans biopsies. B afzelii (51%) and B. garinii (27%) were the most common in ticks. From the 44 sequences obtained from erythema migrans samples 42 were B. afzelii, one B. garinii and one B. bavariensis. Significant associations with erythema migrans formation were found for four IGS and two ospC types. Five from 45 ospC types were associated with more than one genospecies.

Conclusions: B. burgdorferi sl isolates differ in their propensity to cause erythema migrans. These differences were also found within genospecies. In other words, although B. afzelii was mostly associated with erythema migrans, some B. afzelii isolates had a low ability to cause erythema migrans. Our data further support the occurrence of plasmid exchange between borrelia genospecies under natural conditions.

Citing Articles

Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans.

Kapo N, Zuber Bogdanovic I, Gagovic E, Zekic M, Veinovic G, Sukara R Parasit Vectors. 2024; 17(1):45.

PMID: 38297327 PMC: 10832161. DOI: 10.1186/s13071-023-06116-1.

Lyme Disease in Humans.

Radolf J, Strle K, Lemieux J, Strle F Curr Issues Mol Biol. 2020; 42:333-384.

PMID: 33303701 PMC: 7946767. DOI: 10.21775/cimb.042.333.

Chronic Lyme Disease: An Evidence-Based Definition by the ILADS Working Group.

Shor S, Green C, Szantyr B, Phillips S, Liegner K, Burrascano Jr J Antibiotics (Basel). 2020; 8(4).

PMID: 31888310 PMC: 6963229. DOI: 10.3390/antibiotics8040269.

Lyme Disease Frontiers: Reconciling Biology and Clinical Conundrums.

Bamm V, Ko J, Mainprize I, Sanderson V, Wills M Pathogens. 2020; 8(4).

PMID: 31888245 PMC: 6963551. DOI: 10.3390/pathogens8040299.

Precision medicine: retrospective chart review and data analysis of 200 patients on dapsone combination therapy for chronic Lyme disease/post-treatment Lyme disease syndrome: part 1.

Horowitz R, Freeman P Int J Gen Med. 2019; 12:101-119.

PMID: 30863136 PMC: 6388746. DOI: 10.2147/IJGM.S193608.

References

Picken R, Strle F, Picken M, Ruzic-Sabljic E, Maraspin V, Lotric-Furlan S . Identification of three species of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. garinii, and B. afzelii) among isolates from acrodermatitis chronica atrophicans lesions. J Invest Dermatol. 1998; 110(3):211-4. DOI: 10.1046/j.1523-1747.1998.00130.x. View

Flicek B . Rickettsial and other tick-borne infections. Crit Care Nurs Clin North Am. 2007; 19(1):27-38. DOI: 10.1016/j.ccell.2006.11.001. View

Seinost G, Dykhuizen D, Dattwyler R, Golde W, Dunn J, Wang I . Four clones of Borrelia burgdorferi sensu stricto cause invasive infection in humans. Infect Immun. 1999; 67(7):3518-24. PMC: 116539. DOI: 10.1128/IAI.67.7.3518-3524.1999. View

Heylen D, Tijsse E, Fonville M, Matthysen E, Sprong H . Transmission dynamics of Borrelia burgdorferi s.l. in a bird tick community. Environ Microbiol. 2013; 15(2):663-73. DOI: 10.1111/1462-2920.12059. View

Rudenko N, Golovchenko M, Grubhoffer L, Oliver Jr J . Updates on Borrelia burgdorferi sensu lato complex with respect to public health. Ticks Tick Borne Dis. 2011; 2(3):123-8. PMC: 3167092. DOI: 10.1016/j.ttbdis.2011.04.002. View

Tijsse-Klasen E, Fonville M, Reimerink J, Spitzen-van der Sluijs A, Sprong H . Role of sand lizards in the ecology of Lyme and other tick-borne diseases in the Netherlands. Parasit Vectors. 2010; 3:42. PMC: 2890652. DOI: 10.1186/1756-3305-3-42. View

Dykhuizen D, Brisson D, Sandigursky S, Wormser G, Nowakowski J, Nadelman R . The propensity of different Borrelia burgdorferi sensu stricto genotypes to cause disseminated infections in humans. Am J Trop Med Hyg. 2008; 78(5):806-10. PMC: 2387051. View

Aucott J, Seifter A, Rebman A . Probable late lyme disease: a variant manifestation of untreated Borrelia burgdorferi infection. BMC Infect Dis. 2012; 12:173. PMC: 3449205. DOI: 10.1186/1471-2334-12-173. View

Ryffel K, Peter O, Rutti B, SUARD A, Dayer E . Scored antibody reactivity determined by immunoblotting shows an association between clinical manifestations and presence of Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, and B. Valaisiana in humans. J Clin Microbiol. 1999; 37(12):4086-92. PMC: 85886. DOI: 10.1128/JCM.37.12.4086-4092.1999. View

10.

Sarkar A, Hayes B, Dulebohn D, Rosa P . Regulation of the virulence determinant OspC by bbd18 on linear plasmid lp17 of Borrelia burgdorferi. J Bacteriol. 2011; 193(19):5365-73. PMC: 3187453. DOI: 10.1128/JB.01496-10. View

11.

Kenedy M, Lenhart T, Akins D . The role of Borrelia burgdorferi outer surface proteins. FEMS Immunol Med Microbiol. 2012; 66(1):1-19. PMC: 3424381. DOI: 10.1111/j.1574-695X.2012.00980.x. View

12.

Liegl G, Preac-Mursic V, Rossler D, Schwab E, Soutschek E, WILL G . Molecular analysis of genes encoding outer surface protein C (OspC) of Borrelia burgdorferi sensu lato: relationship to ospA genotype and evidence of lateral gene exchange of ospC. J Clin Microbiol. 1995; 33(7):1860-6. PMC: 228286. DOI: 10.1128/jcm.33.7.1860-1866.1995. View

13.

Wang I, Dykhuizen D, Qiu W, Dunn J, Bosler E, Luft B . Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto. Genetics. 1999; 151(1):15-30. PMC: 1460459. DOI: 10.1093/genetics/151.1.15. View

14.

DICKMAN S . Possible cause found for Lyme arthritis. Science. 1998; 281(5377):631-2. DOI: 10.1126/science.281.5377.631. View

15.

Masuzawa T, Komikado T, Iwaki A, Suzuki H, Kaneda K, Yanagihara Y . Characterization of Borrelia sp. isolated from Ixodes tanuki, I. turdus, and I. columnae in Japan by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. FEMS Microbiol Lett. 1996; 142(1):77-83. DOI: 10.1111/j.1574-6968.1996.tb08411.x. View

16.

Ornstein K, Berglund J, Bergstrom S, Norrby R, Barbour A . Three major Lyme Borrelia genospecies (Borrelia burgdorferi sensu stricto, B. afzelii and B. garinii) identified by PCR in cerebrospinal fluid from patients with neuroborreliosis in Sweden. Scand J Infect Dis. 2002; 34(5):341-6. DOI: 10.1080/00365540110080313. View

17.

Margos G, Vollmer S, Ogden N, Fish D . Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato. Infect Genet Evol. 2011; 11(7):1545-63. PMC: 3214628. DOI: 10.1016/j.meegid.2011.07.022. View

18.

Grimm D, Tilly K, Byram R, Stewart P, Krum J, Bueschel D . Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals. Proc Natl Acad Sci U S A. 2004; 101(9):3142-7. PMC: 365757. DOI: 10.1073/pnas.0306845101. View

19.

Schaarschmidt D, Oehme R, Kimmig P, Hesch R, Englisch S . Detection and molecular typing of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks and in different patient samples from southwest Germany. Eur J Epidemiol. 2003; 17(12):1067-74. DOI: 10.1023/a:1021286528058. View

20.

Egyed L, Elo P, Sreter-Lancz Z, Szell Z, Balogh Z, Sreter T . Seasonal activity and tick-borne pathogen infection rates of Ixodes ricinus ticks in Hungary. Ticks Tick Borne Dis. 2012; 3(2):90-4. DOI: 10.1016/j.ttbdis.2012.01.002. View