Scanning Ion Conductance Microscopy: a Nanotechnology for Biological Studies in Live Cells

Overview

Journal Front Physiol

Date 2013 Jan 22

PMID 23335899

Citations 8

Authors

Bing-Chen Liu

Xiao-Yu Lu

Xiang Song

Ke-Yu Lei

Abdel A Alli

Hui-Fang Bao

Douglas C Eaton

He-Ping Ma

Affiliations

Soon will be listed here.

Abstract

Scanning ion-conductance microscope (SICM), which enables high-resolution imaging of cell surface topography, has been developed for over two decades. However, only recently, a unique scanning mode is increasingly used in biological studies to allow SICM to detect the surface of live cells. More recently, in combination with confocal microscopy and patch-clamp electrophysiological techniques, SICM allows investigators to localize proteins or ion channels in a specific nanostructure at the cell surface. This article will briefly review SICM nanotechnique and summarize the role of SICM in biological studies.

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