Quantitative Analysis of Muscle Histologic Method in Rodent Facial Nerve Injury

Overview

Journal JAMA Facial Plast Surg

Publisher American Medical Association

Date 2013 Jan 19

PMID 23329158

Citations 4

Authors

Tessa A Hadlock

Sang W Kim

Julie S Weinberg

Christopher J Knox

Marc H Hohman

James T Heaton

Affiliations

Soon will be listed here.

Abstract

Objective: To describe denervation features of facial musculature following facial nerve injury in a rodent model.

Methods: Six Wistar-Hannover rats underwent unilateral transection and immediate repair of the facial nerve. After 8 weeks, muscular bundles consisting of dilator naris and levator labii superioris from both sides were analyzed for mean muscle cell diameter and the percentage of muscle cell cross-sectional area using image processing software. The atrophic features of facial muscles were quantified and compared with the contralateral, healthy side of the face.

Results: Weekly postoperative whisking assessment demonstrated the anticipated course of recovery. We observed significant differences between the normal side and the manipulated side, respectively, in the percentage of muscle specimen cross-sectional area attributable to muscle cell profiles (57% vs 29%; P = .006) and total fiber counts (1346 vs 794; P = .02). The mean cross-sectional area of individual muscle fibers was higher on the normal side (1129 vs 928 μm2; P = .39); however, this difference was statistically nonsignificant.

Conclusion: The objective, quantitative measures of muscle microstructure used in this report provide a valuable point of comparison for whisking function and electrophysiologic measures and can be used in future studies to assess muscle atrophic features associated with facial nerve injury and repair techniques.

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