Characterization of Halobacterium Halobium Mutants Defective in Taxis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 May 1

PMID 2332402

Citations 13

Authors

S A. Sundberg

M Alam

M Lebert

J L Spudich

D Oesterhelt

G L Hazelbauer

Affiliations

Soon will be listed here.

Abstract

Mutant derivatives of Halobacterium halobium previously isolated by using a procedure that selected for defective phototactic response to white light were examined for an array of phenotypic characteristics related to phototaxis and chemotaxis. The properties tested were unstimulated swimming behavior, behaviorial responses to temporal gradients of light and spatial gradients of chemoattractants, content of photoreceptor pigments, methylation of methyl-accepting taxis proteins, and transient increases in rate of release of volatile methyl groups induced by tactic stimulation. Several distinct phenotypes were identified, corresponding to a mutant missing photoreceptors, a mutant defective in the methyltransferase, a mutant altered in control of the methylesterase, and mutants apparently defective in intracellular signaling. All except the photoreceptor mutant were defective in both chemotaxis and phototaxis.

Citing Articles

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A predictive computational model of the kinetic mechanism of stimulus-induced transducer methylation and feedback regulation through CheY in archaeal phototaxis and chemotaxis.

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Evolutionary conservation of methyl-accepting chemotaxis protein location in Bacteria and Archaea.

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Constitutive signaling by the phototaxis receptor sensory rhodopsin II from disruption of its protonated Schiff base-Asp-73 interhelical salt bridge.

Spudich E, Zhang W, Alam M, Spudich J Proc Natl Acad Sci U S A. 1997; 94(10):4960-5.

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References

MACDONALD R, Lanyi L . Light-induced leucine transport in Halobacterium halobium envelope vesicles: a chemiosmotic system. Biochemistry. 1975; 14(13):2882-9. DOI: 10.1021/bi00684a014. View

Otomo J, Marwan W, Oesterhelt D, Desel H, Uhl R . Biosynthesis of the two halobacterial light sensors P480 and sensory rhodopsin and variation in gain of their signal transduction chains. J Bacteriol. 1989; 171(4):2155-9. PMC: 209871. DOI: 10.1128/jb.171.4.2155-2159.1989. View

PARKINSON J, Revello P . Sensory adaptation mutants of E. coli. Cell. 1978; 15(4):1221-30. DOI: 10.1016/0092-8674(78)90048-x. View

Schimz A, Hildebrand E . Chemosensory responses of Halobacterium halobium. J Bacteriol. 1979; 140(3):749-53. PMC: 216711. DOI: 10.1128/jb.140.3.749-753.1979. View

Schimz A . Methylation of membrane proteins is involved in chemosensory and photosensory behavior of Halobacterium halobium. FEBS Lett. 1981; 125(2):205-7. DOI: 10.1016/0014-5793(81)80719-3. View

PARKINSON J, Houts S . Isolation and behavior of Escherichia coli deletion mutants lacking chemotaxis functions. J Bacteriol. 1982; 151(1):106-13. PMC: 220204. DOI: 10.1128/jb.151.1.106-113.1982. View

Spudich E, Spudich J . Control of transmembrane ion fluxes to select halorhodopsin-deficient and other energy-transduction mutants of Halobacterium halobium. Proc Natl Acad Sci U S A. 1982; 79(14):4308-12. PMC: 346660. DOI: 10.1073/pnas.79.14.4308. View

Bogomolni R, Spudich J . Identification of a third rhodopsin-like pigment in phototactic Halobacterium halobium. Proc Natl Acad Sci U S A. 1982; 79(20):6250-4. PMC: 347098. DOI: 10.1073/pnas.79.20.6250. View

Kehry M, Dahlquist F . Adaptation in bacterial chemotaxis: CheB-dependent modification permits additional methylations of sensory transducer proteins. Cell. 1982; 29(3):761-72. DOI: 10.1016/0092-8674(82)90438-x. View

10.

PARKINSON J, Parker S, Talbert P, Houts S . Interactions between chemotaxis genes and flagellar genes in Escherichia coli. J Bacteriol. 1983; 155(1):265-74. PMC: 217677. DOI: 10.1128/jb.155.1.265-274.1983. View

11.

Kehry M, Doak T, Dahlquist F . Stimulus-induced changes in methylesterase activity during chemotaxis in Escherichia coli. J Biol Chem. 1984; 259(19):11828-35. View

12.

Ehrlich B, Schen C, Spudich J . Bacterial rhodopsins monitored with fluorescent dyes in vesicles and in vivo. J Membr Biol. 1984; 82(1):89-94. DOI: 10.1007/BF01870735. View

13.

Spudich J, Bogomolni R . Mechanism of colour discrimination by a bacterial sensory rhodopsin. Nature. 1984; 312(5994):509-13. PMC: 4996264. DOI: 10.1038/312509a0. View

14.

Sundberg S, Bogomolni R, Spudich J . Selection and properties of phototaxis-deficient mutants of Halobacterium halobium. J Bacteriol. 1985; 164(1):282-7. PMC: 214241. DOI: 10.1128/jb.164.1.282-287.1985. View

15.

Hildebrand E, Schimz A . Integration of photosensory signals in Halobacterium halobium. J Bacteriol. 1986; 167(1):305-11. PMC: 212876. DOI: 10.1128/jb.167.1.305-311.1986. View

16.

Ravid S, Matsumura P, Eisenbach M . Restoration of flagellar clockwise rotation in bacterial envelopes by insertion of the chemotaxis protein CheY. Proc Natl Acad Sci U S A. 1986; 83(19):7157-61. PMC: 386674. DOI: 10.1073/pnas.83.19.7157. View

17.

Wolff E, Bogomolni R, SCHERRER P, Hess B, Stoeckenius W . Color discrimination in halobacteria: spectroscopic characterization of a second sensory receptor covering the blue-green region of the spectrum. Proc Natl Acad Sci U S A. 1986; 83(19):7272-6. PMC: 386698. DOI: 10.1073/pnas.83.19.7272. View

18.

Tomioka H, Takahashi T, Kamo N, Kobatake Y . Flash spectrophotometric identification of a fourth rhodopsin-like pigment in Halobacterium halobium. Biochem Biophys Res Commun. 1986; 139(2):389-95. DOI: 10.1016/s0006-291x(86)80003-1. View

19.

Sundberg S, Alam M, Spudich J . Excitation signal processing times in Halobacterium halobium phototaxis. Biophys J. 1986; 50(5):895-900. PMC: 1329814. DOI: 10.1016/S0006-3495(86)83530-5. View

20.

Kuo S, Koshland Jr D . Roles of cheY and cheZ gene products in controlling flagellar rotation in bacterial chemotaxis of Escherichia coli. J Bacteriol. 1987; 169(3):1307-14. PMC: 211935. DOI: 10.1128/jb.169.3.1307-1314.1987. View