High Throughput Phenotypic Selection of Mycobacterium Tuberculosis Mutants with Impaired Resistance to Reactive Oxygen Species Identifies Genes Important for Intracellular Growth

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Jan 16

PMID 23320090

Citations 10

Authors

Olga Mestre

Raquel Hurtado-Ortiz

Tiago Dos Vultos

Amine Namouchi

Mena Cimino

Madalena Pimentel

Olivier Neyrolles

Brigitte Gicquel

Affiliations

Soon will be listed here.

Abstract

Mycobacterium tuberculosis has the remarkable capacity to survive within the hostile environment of the macrophage, and to resist potent antibacterial molecules such as reactive oxygen species (ROS). Thus, understanding mycobacterial resistance mechanisms against ROS may contribute to the development of new anti-tuberculosis therapies. Here we identified genes involved in such mechanisms by screening a high-density transposon mutant library, and we show that several of them are involved in the intracellular lifestyle of the pathogen. Many of these genes were found to play a part in cell envelope functions, further strengthening the important role of the mycobacterial cell envelope in protection against aggressions such as the ones caused by ROS inside host cells.

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