Vaccine Protection Against Bacillus Cereus-mediated Respiratory Anthrax-like Disease in Mice

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2013 Jan 16

PMID 23319564

Citations 6

Authors

So-Young Oh

Hannah Maier

Jay Schroeder

G Stefan Richter

Derek Elli

James M Musser

Lauriane E Quenee

Dominique M Missiakas

Olaf Schneewind

Affiliations

Soon will be listed here.

Abstract

Bacillus cereus strains harboring a pXO1-like virulence plasmid cause respiratory anthrax-like disease in humans, particularly in welders. We developed mouse models for intraperitoneal as well as aerosol challenge with spores of B. cereus G9241, harboring pBCXO1 and pBC218 virulence plasmids. Compared to wild-type B. cereus G9241, spores with a deletion of the pBCXO1-carried protective antigen gene (pagA1) were severely attenuated, whereas spores with a deletion of the pBC218-carried protective antigen homologue (pagA2) were not. Anthrax vaccine adsorbed (AVA) immunization raised antibodies that bound and neutralized the pagA1-encoded protective antigen (PA1) but not the PA2 orthologue encoded by pagA2. AVA immunization protected mice against a lethal challenge with spores from B. cereus G9241 or B. cereus Elc4, a strain that had been isolated from a fatal case of anthrax-like disease. As the pathogenesis of B. cereus anthrax-like disease in mice is dependent on pagA1 and PA-neutralizing antibodies provide protection, AVA immunization may also protect humans from respiratory anthrax-like death.

Citing Articles

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Certhrax Is an Antivirulence Factor for the Anthrax-Like Organism Bacillus cereus Strain G9241.

Seldina Y, Petro C, Servetas S, Vergis J, Ventura C, Merrell D Infect Immun. 2018; 86(6).

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Cytotoxic Potential of Bacillus cereus Strains ATCC 11778 and 14579 Against Human Lung Epithelial Cells Under Microaerobic Growth Conditions.

Kilcullen K, Teunis A, Popova T, Popov S Front Microbiol. 2016; 7:69.

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Brezillon C, Haustant M, Dupke S, Corre J, Lander A, Franz T PLoS Negl Trop Dis. 2015; 9(4):e0003455.

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