Comparative Evaluation of Vitek 2 Identification and Susceptibility Testing of Urinary Tract Pathogens Directly and Isolated from Chromogenic Media

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2013 Jan 15

PMID 23314701

Citations 6

Authors

M J Munoz-Davila

M Roig

G Yague

A Blazquez

C Salvador

M Segovia

Affiliations

Soon will be listed here.

Abstract

We evaluated the use of urine specimens for direct identification and antibiotic testing of urinary tract pathogens using the Vitek system. A total of 343 urine specimens from patients with suspected UTI were selected by pyuria and screened by Gram staining to detect bacteriuria. Of those, 132 were analysed after Gram staining, showing a high number of micro-organisms of a single morphological type. Direct susceptibility testing and identification were performed by using the Vitek system. Results were compared using the standard inoculation method based on the incubation of solid media. After sub-culture, 107 specimens grew a significant count of a single species and were used for the comparative analysis. The direct method correctly identified 88 isolates (82.3 %). When compared according to antibiotic susceptibility testing, the error rate was 2.4 % overall with 0.2 % very major, 0.4 % major and 1.8 % minor errors. 84.7 % of the Gram-negative bacilli had a complete susceptibility report in ≤ 8 h. This method offers the advantage of prompt processing and earlier reporting of complete results for positive urine specimens.

Citing Articles

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Direct identification and susceptibility testing of Gram-negative bacilli from turbid urine samples using VITEK2.

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A general method for rapid determination of antibiotic susceptibility and species in bacterial infections.

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Evaluation of two chromogenic media for the isolation and identification of urinary tract pathogens.

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References

Bizzini A, Durussel C, Bille J, Greub G, Prodhom G . Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J Clin Microbiol. 2010; 48(5):1549-54. PMC: 2863943. DOI: 10.1128/JCM.01794-09. View

Ilki A, Bekdemir P, Ulger N, Soyletir G . Rapid reporting of urine culture results: impact of the uro-quick screening system. New Microbiol. 2010; 33(2):147-53. View

Munoz-Davila M, Yague G, Albert M, Garcia-Lucas T . Comparative evaluation of Vitek 2 identification and susceptibility testing of Gram-negative rods directly and isolated from BacT/ALERT-positive blood culture bottles. Eur J Clin Microbiol Infect Dis. 2011; 31(5):663-9. DOI: 10.1007/s10096-011-1356-1. View

MacGowan A . Clinical implications of antimicrobial resistance for therapy. J Antimicrob Chemother. 2008; 62 Suppl 2:ii105-14. DOI: 10.1093/jac/dkn357. View

Wellstood S . Direct identification and susceptibility testing by the AutoMicrobic system of gram-negative bacilli from urine specimens. J Clin Microbiol. 1986; 23(6):1068-71. PMC: 268795. DOI: 10.1128/jcm.23.6.1068-1071.1986. View

Doern G, Brueggemann A, Perla R, Daly J, HALKIAS D, Jones R . Multicenter laboratory evaluation of the bioMérieux Vitek antimicrobial susceptibility testing system with 11 antimicrobial agents versus members of the family Enterobacteriaceae and Pseudomonas aeruginosa. J Clin Microbiol. 1997; 35(8):2115-9. PMC: 229914. DOI: 10.1128/jcm.35.8.2115-2119.1997. View

Ling T, Tam P, Liu Z, Cheng A . Evaluation of VITEK 2 rapid identification and susceptibility testing system against gram-negative clinical isolates. J Clin Microbiol. 2001; 39(8):2964-6. PMC: 88270. DOI: 10.1128/JCM.39.8.2964-2966.2001. View

Pezzlo M . Detection of urinary tract infections by rapid methods. Clin Microbiol Rev. 1988; 1(3):268-80. PMC: 358050. DOI: 10.1128/CMR.1.3.268. View

Peleg A, Hooper D . Hospital-acquired infections due to gram-negative bacteria. N Engl J Med. 2010; 362(19):1804-13. PMC: 3107499. DOI: 10.1056/NEJMra0904124. View

10.

Quesada M, Gimenez M, Molinos S, Fernandez G, Sanchez M, Rivelo R . Performance of VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing of Gram-negative bacilli from positive FAN BacT/ALERT blood culture bottles. Clin Microbiol Infect. 2009; 16(2):137-40. DOI: 10.1111/j.1469-0691.2009.02907.x. View

11.

Broeren M, Bahceci S, Vader H, Arents N . Screening for urinary tract infection with the Sysmex UF-1000i urine flow cytometer. J Clin Microbiol. 2011; 49(3):1025-9. PMC: 3067737. DOI: 10.1128/JCM.01669-10. View

12.

Ferreira L, Sanchez-Juanes F, Gonzalez-Avila M, Cembrero-Fucinos D, Herrero-Hernandez A, Gonzalez-Buitrago J . Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2010; 48(6):2110-5. PMC: 2884468. DOI: 10.1128/JCM.02215-09. View

13.

GRAHAM J, Galloway A . ACP Best Practice No 167: the laboratory diagnosis of urinary tract infection. J Clin Pathol. 2001; 54(12):911-9. PMC: 1731340. DOI: 10.1136/jcp.54.12.911. View

14.

Ling T, Liu Z, Cheng A . Evaluation of the VITEK 2 system for rapid direct identification and susceptibility testing of gram-negative bacilli from positive blood cultures. J Clin Microbiol. 2003; 41(10):4705-7. PMC: 254354. DOI: 10.1128/JCM.41.10.4705-4707.2003. View

15.

Ferreira L, Vega S, Sanchez-Juanes F, Gonzalez M, Herrero A, Muniz M . [Identifying bacteria using a matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer. Comparison with routine methods used in clinical microbiology laboratories]. Enferm Infecc Microbiol Clin. 2010; 28(8):492-7. DOI: 10.1016/j.eimc.2009.12.009. View

16.

Kohling H, Bittner A, Muller K, Buer J, Becker M, Rubben H . Direct identification of bacteria in urine samples by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and relevance of defensins as interfering factors. J Med Microbiol. 2012; 61(Pt 3):339-344. DOI: 10.1099/jmm.0.032284-0. View

17.

Lamb V, Dalton H, WILKINS J . Electrochemical method for the early detection of urinary-tract infections. Am J Clin Pathol. 1976; 66(1):91-5. DOI: 10.1093/ajcp/66.1.91. View

18.

Jorgensen J, Ferraro M . Antimicrobial susceptibility testing: general principles and contemporary practices. Clin Infect Dis. 1998; 26(4):973-80. DOI: 10.1086/513938. View

19.

Foxman B . Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Am J Med. 2002; 113 Suppl 1A:5S-13S. DOI: 10.1016/s0002-9343(02)01054-9. View

20.

Galar A, Yuste J, Espinosa M, Guillen-Grima F, Hernaez-Crespo S, Leiva J . Clinical and economic impact of rapid reporting of bacterial identification and antimicrobial susceptibility results of the most frequently processed specimen types. Eur J Clin Microbiol Infect Dis. 2012; 31(9):2445-52. DOI: 10.1007/s10096-012-1588-8. View