Metagenomic Analysis of Healthy and White Plague-affected Mussismilia Braziliensis Corals

Overview

Journal Microb Ecol

Specialties Environmental Health
Microbiology

Date 2013 Jan 15

PMID 23314124

Citations 42

Authors

Gizele D Garcia

Gustavo B Gregoracci

Eidy de O Santos

Pedro M Meirelles

Genivaldo G Z Silva

Rob Edwards

Tomoo Sawabe

Kazuyoshi Gotoh

Shota Nakamura

Tetsuya Iida

Rodrigo L de Moura

Fabiano L Thompson

Affiliations

Soon will be listed here.

Abstract

Coral health is under threat throughout the world due to regional and global stressors. White plague disease (WP) is one of the most important threats affecting the major reef builder of the Abrolhos Bank in Brazil, the endemic coral Mussismilia braziliensis. We performed a metagenomic analysis of healthy and WP-affected M. braziliensis in order to determine the types of microbes associated with this coral species. We also optimized a protocol for DNA extraction from coral tissues. Our taxonomic analysis revealed Proteobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, and Actinomycetes as the main groups in all healthy and WP-affected corals. Vibrionales, members of the Cytophaga-Flavobacterium-Bacteroides complex, Rickettsiales, and Neisseriales were more abundant in the WP-affected corals. Diseased corals also had more eukaryotic metagenomic sequences identified as Alveolata and Apicomplexa. Our results suggest that WP disease in M. braziliensis is caused by a polymicrobial consortium.

Citing Articles

The Abrolhos Nominally Herbivorous Coral Reef Fish Acanthurus chirurgus, Kyphosus sp., Scarus trispinosus, and Sparisoma axillare Have Similarities in Feeding But Species-Specific Microbiomes.

Thompson C, Silva R, Gibran F, Bacha L, de Freitas M, Thompson M Microb Ecol. 2024; 87(1):110.

PMID: 39215820 PMC: 11365853. DOI: 10.1007/s00248-024-02423-x.

The Impact of Highly Weathered Oil from the Most Extensive Oil Spill in Tropical Oceans (Brazil) on the Microbiome of the Coral .

Pereira P, Fernandes L, Jesus H, Costa P, Lacerda C, Mies M Microorganisms. 2023; 11(8).

PMID: 37630495 PMC: 10458584. DOI: 10.3390/microorganisms11081935.

Meta-analysis reveals variations in microbial communities from diverse stony coral taxa at different geographical distances.

Pei P, Liu L, Jing X, Liu X, Sun L, Gao C Front Microbiol. 2023; 14:1087750.

PMID: 37520377 PMC: 10374221. DOI: 10.3389/fmicb.2023.1087750.

Host-specific symbioses and the microbial prey of a pelagic tunicate (Pyrosoma atlanticum).

Thompson A, Ward A, Sweeney C, Sutherland K ISME Commun. 2023; 1(1):11.

PMID: 36721065 PMC: 9723572. DOI: 10.1038/s43705-021-00007-1.

Endophytic fungus produces cyclopeptides and plant-related bioactive rotenoids.

Sobreira A, Pinto F, Florencio K, Wilke D, Staats C, Streit R RSC Adv. 2022; 8(62):35575-35586.

PMID: 35547902 PMC: 9088075. DOI: 10.1039/c8ra06824k.

References

Gardner T, Cote I, Gill J, Grant A, Watkinson A . Long-term region-wide declines in Caribbean corals. Science. 2003; 301(5635):958-60. DOI: 10.1126/science.1086050. View

Austin B, Austin D, Sutherland R, Thompson F, Swings J . Pathogenicity of vibrios to rainbow trout (Oncorhynchus mykiss, Walbaum) and Artemia nauplii. Environ Microbiol. 2005; 7(9):1488-95. DOI: 10.1111/j.1462-2920.2005.00847.x. View

Bentis C, Kaufman L, Golubic S . Endolithic fungi in reef-building corals (Order : Scleractinia) are common, cosmopolitan, and potentially pathogenic. Biol Bull. 2000; 198(2):254-60. DOI: 10.2307/1542528. View

Faulkner G, Kimura Y, Daub C, Wani S, Plessy C, Irvine K . The regulated retrotransposon transcriptome of mammalian cells. Nat Genet. 2009; 41(5):563-71. DOI: 10.1038/ng.368. View

Coufal N, Garcia-Perez J, Peng G, Yeo G, Mu Y, Lovci M . L1 retrotransposition in human neural progenitor cells. Nature. 2009; 460(7259):1127-31. PMC: 2909034. DOI: 10.1038/nature08248. View

Pantos O, Cooney R, Le Tissier M, Barer M, ODonnell A, Bythell J . The bacterial ecology of a plague-like disease affecting the Caribbean coral Montastrea annularis. Environ Microbiol. 2003; 5(5):370-82. DOI: 10.1046/j.1462-2920.2003.00427.x. View

Parks D, Beiko R . Identifying biologically relevant differences between metagenomic communities. Bioinformatics. 2010; 26(6):715-21. DOI: 10.1093/bioinformatics/btq041. View

Willner D, Thurber R, Rohwer F . Metagenomic signatures of 86 microbial and viral metagenomes. Environ Microbiol. 2009; 11(7):1752-66. DOI: 10.1111/j.1462-2920.2009.01901.x. View

Sunagawa S, DeSantis T, Piceno Y, Brodie E, DeSalvo M, Voolstra C . Bacterial diversity and White Plague Disease-associated community changes in the Caribbean coral Montastraea faveolata. ISME J. 2009; 3(5):512-21. DOI: 10.1038/ismej.2008.131. View

10.

Madin J, Connolly S . Ecological consequences of major hydrodynamic disturbances on coral reefs. Nature. 2006; 444(7118):477-80. DOI: 10.1038/nature05328. View

11.

Shinzato C, Shoguchi E, Kawashima T, Hamada M, Hisata K, Tanaka M . Using the Acropora digitifera genome to understand coral responses to environmental change. Nature. 2011; 476(7360):320-3. DOI: 10.1038/nature10249. View

12.

Godwin S, Bent E, Borneman J, Pereg L . The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina. PLoS One. 2012; 7(9):e44243. PMC: 3435329. DOI: 10.1371/journal.pone.0044243. View

13.

Macfarlan T, Gifford W, Driscoll S, Lettieri K, Rowe H, Bonanomi D . Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature. 2012; 487(7405):57-63. PMC: 3395470. DOI: 10.1038/nature11244. View

14.

Kuznetsov Y, McPherson A . Atomic force microscopy in imaging of viruses and virus-infected cells. Microbiol Mol Biol Rev. 2011; 75(2):268-85. PMC: 3122623. DOI: 10.1128/MMBR.00041-10. View

15.

Cypriano-Souza A, Fernandez G, Lima-Rosa C, Engel M, Bonatto S . Microsatellite genetic characterization of the humpback whale (Megaptera novaeangliae) breeding ground off Brazil (breeding stock A). J Hered. 2009; 101(2):189-200. DOI: 10.1093/jhered/esp097. View

16.

Gladyshev E, Meselson M, Arkhipova I . A deep-branching clade of retrovirus-like retrotransposons in bdelloid rotifers. Gene. 2006; 390(1-2):136-45. PMC: 1839950. DOI: 10.1016/j.gene.2006.09.025. View

17.

Kvennefors E, Sampayo E, Ridgway T, Barnes A, Hoegh-Guldberg O . Bacterial communities of two ubiquitous Great Barrier Reef corals reveals both site- and species-specificity of common bacterial associates. PLoS One. 2010; 5(4):e10401. PMC: 2861602. DOI: 10.1371/journal.pone.0010401. View

18.

Meyer F, Paarmann D, DSouza M, Olson R, Glass E, Kubal M . The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC Bioinformatics. 2008; 9:386. PMC: 2563014. DOI: 10.1186/1471-2105-9-386. View

19.

McDole T, Nulton J, Barott K, Felts B, Hand C, Hatay M . Assessing coral reefs on a Pacific-wide scale using the microbialization score. PLoS One. 2012; 7(9):e43233. PMC: 3436891. DOI: 10.1371/journal.pone.0043233. View

20.

Gregoracci G, Nascimento J, Cabral A, Paranhos R, Valentin J, Thompson C . Structuring of bacterioplankton diversity in a large tropical bay. PLoS One. 2012; 7(2):e31408. PMC: 3283626. DOI: 10.1371/journal.pone.0031408. View