Systems Genetics Implicates Cytoskeletal Genes in Oocyte Control of Cloned Embryo Quality

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2013 Jan 12

PMID 23307892

Citations 8

Authors

Yong Cheng

John Gaughan

Uros Midic

Zhiming Han

Cheng-Guang Liang

Bela G Patel

Keith E Latham

Affiliations

Soon will be listed here.

Abstract

Cloning by somatic cell nuclear transfer is an important technology, but remains limited due to poor rates of success. Identifying genes supporting clone development would enhance our understanding of basic embryology, improve applications of the technology, support greater understanding of establishing pluripotent stem cells, and provide new insight into clinically important determinants of oocyte quality. For the first time, a systems genetics approach was taken to discover genes contributing to the ability of an oocyte to support early cloned embryo development. This identified a primary locus on mouse chromosome 17 and potential loci on chromosomes 1 and 4. A combination of oocyte transcriptome profiling data, expression correlation analysis, and functional and network analyses yielded a short list of likely candidate genes in two categories. The major category-including two genes with the strongest genetic associations with the traits (Epb4.1l3 and Dlgap1)-encodes proteins associated with the subcortical cytoskeleton and other cytoskeletal elements such as the spindle. The second category encodes chromatin and transcription regulators (Runx1t1, Smchd1, and Chd7). Smchd1 promotes X chromosome inactivation, whereas Chd7 regulates expression of pluripotency genes. Runx1t1 has not been associated with these processes, but acts as a transcriptional repressor. The finding that cytoskeleton-associated proteins may be key determinants of early clone development highlights potential roles for cytoplasmic components of the oocyte in supporting nuclear reprogramming. The transcriptional regulators identified may contribute to the overall process as downstream effectors.

Citing Articles

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Genotypic divergence in mouse oocyte transcriptomes: possible pathways to hybrid vigor impacting fertility and embryogenesis.

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SMCHD1 terminates the first embryonic genome activation event in mouse two-cell embryos and contributes to a transcriptionally repressive state.

Ruebel M, Vincent K, Schall P, Wang K, Latham K Am J Physiol Cell Physiol. 2019; 317(4):C655-C664.

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Novel key roles for structural maintenance of chromosome flexible domain containing 1 (Smchd1) during preimplantation mouse development.

Midic U, Vincent K, Wang K, Lokken A, Severance A, Ralston A Mol Reprod Dev. 2018; 85(7):635-648.

PMID: 29900695 PMC: 6361378. DOI: 10.1002/mrd.23001.

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