Rapid Procedure to Determine the DNA Base Composition from Small Amounts of Gram-positive Bacteria

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 1990 Jan 15

PMID 2328905

Citations 7

Authors

T Ezaki

S M Saidi

S L Liu

Y Hashimoto

H Yamamoto

E Yabuuchi

Affiliations

Soon will be listed here.

Abstract

A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromo-peptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h.

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