Prevalence and Characterization of Genotypic Diversity of Haemophilus Parasuis Isolates from Southern China

Overview

Journal Can J Vet Res

Specialty Veterinary Medicine

Date 2013 Jan 2

PMID 23277703

Citations 14

Authors

Jianmin Zhang

Chenggang Xu

Lili Guo

Haiyan Shen

Xiaoling Deng

Changwen Ke

Bixia Ke

Bin Zhang

Ang Li

Tao Ren

Ming Liao

Affiliations

Soon will be listed here.

Abstract

From September 2008 to December 2010, 112 Haemophilus parasuis strains were isolated from 536 pigs with clinical signs of Glässer's disease in South China, for a frequency of 21%. The 112 strains were subjected to serovar analysis by gel diffusion (GD) and indirect hemagglutination (IHA) tests and to genotype analysis by means of pulsed-field gel electrophoresis (PFGE). With a combination of the GD and IHA results, serovars 5 and 4 were found to be the most prevalent, at 23% and 17%, respectively, followed by serovars 2 (8%), 15 (7%), 13 (6%), and 12 (5%); 20% of the strains were nontypeable. The 112 strains were genetically diverse, with 85 genotypes identified (discriminatory index 0.992). The 89 typeable isolates belonged to 15 H. parasuis serovars displaying 63 different PFGE profiles. The 23 nontypeable strains displayed 22 different PFGE profiles. These findings confirmed that 15 serovars and diverse genotypes of H. parasuis were widely distributed in southern China.

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