Hyperbaric Oxygenation Alters Temporal Expression Pattern of Superoxide Dismutase 2 After Cortical Stab Injury in Rats

Overview

Journal Croat Med J

Specialty General Medicine

Date 2013 Jan 1

PMID 23275324

Citations 7

Authors

Ana B Parabucki

Iva D Bozic

Ivana M Bjelobaba

Irena C Lavrnja

Predrag D Brkic

Tomislav S Jovanovic

Danijela Z Savic

Mirjana B Stojiljkovic

Sanja M Pekovic

Affiliations

Soon will be listed here.

Abstract

Aim: To evaluate the effect of hyperbaric oxygen therapy (HBOT) on superoxide dismutase 2 (SOD2) expression pattern after the cortical stab injury (CSI).

Methods: CSI was performed on 88 male Wistar rats, divided into control, sham, lesioned, and HBO groups. HBOT protocol was the following: pressure applied was 2.5 absolute atmospheres, for 60 minutes, once a day for consecutive 3 or 10 days. The pattern of SOD2 expression and cellular localization was analyzed using real-time polymerase chain reaction, Western blot, and double-label fluorescence immunohistochemistry. Neurons undergoing degeneration were visualized with Fluoro-Jade®B.

Results: CSI induced significant transient increase in SOD2 protein levels at day 3 post injury, which was followed by a reduction toward control levels at post-injury day 10. At the same time points, mRNA levels for SOD2 in the injured cortex were down-regulated. Exposure to HBO for 3 days considerably down-regulated SOD2 protein levels in the injured cortex, while after 10 days of HBOT an up-regulation of SOD2 was observed. HBOT significantly increased mRNA levels for SOD2 at both time points compared to the corresponding L group, but they were still lower than in controls. Double immunofluorescence staining revealed that 3 days after CSI, up-regulation of SOD2 was mostly due to an increased expression in reactive astrocytes surrounding the lesion site. HBOT attenuated SOD2 expression both in neuronal and astroglial cells. Fluoro-Jade®B labeling showed that HBOT significantly decreased the number of degenerating neurons in the injured cortex.

Conclusion: HBOT alters SOD2 protein and mRNA levels after brain injury in a time-dependent manner.

Citing Articles

Effect of Hyperbaric Oxygen and Inflammation on Human Gingival Mesenchymal Stem/Progenitor Cells.

Tolle J, Koch A, Schlicht K, Finger D, Kaehler W, Hoppner M Cells. 2023; 12(20).

PMID: 37887323 PMC: 10605813. DOI: 10.3390/cells12202479.

Oxygen Variations-Insights into Hypoxia, Hyperoxia and Hyperbaric Hyperoxia-Is the Dose the Clue?.

Balestra C, Mrakic-Sposta S, Virgili F Int J Mol Sci. 2023; 24(17).

PMID: 37686277 PMC: 10488080. DOI: 10.3390/ijms241713472.

Hyperbaric Oxygenation Prevents Loss of Immature Neurons in the Adult Hippocampal Dentate Gyrus Following Brain Injury.

Jeremic R, Pekovic S, Lavrnja I, Bjelobaba I, Djelic M, Dacic S Int J Mol Sci. 2023; 24(5).

PMID: 36901691 PMC: 10002298. DOI: 10.3390/ijms24054261.

Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats.

Kovacevic S, Ivanov M, Zivotic M, Brkic P, Miloradovic Z, Jeremic R Antioxidants (Basel). 2021; 10(8).

PMID: 34439411 PMC: 8388865. DOI: 10.3390/antiox10081163.

Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats.

Kovacevic S, Ivanov M, Miloradovic Z, Brkic P, Vajic U, Zivotic M PLoS One. 2020; 15(1):e0226974.

PMID: 31914135 PMC: 6948727. DOI: 10.1371/journal.pone.0226974.

References

Methy D, Bertrand N, Prigent-Tessier A, Stanimirovic D, Beley A, Marie C . Differential MnSOD and HO-1 expression in cerebral endothelial cells in response to sublethal oxidative stress. Brain Res. 2004; 1003(1-2):151-8. DOI: 10.1016/j.brainres.2003.12.031. View

Masel B, DeWitt D . Traumatic brain injury: a disease process, not an event. J Neurotrauma. 2010; 27(8):1529-40. DOI: 10.1089/neu.2010.1358. View

Clausen F, Lundqvist H, Ekmark S, Lewen A, Ebendal T, Hillered L . Oxygen free radical-dependent activation of extracellular signal-regulated kinase mediates apoptosis-like cell death after traumatic brain injury. J Neurotrauma. 2004; 21(9):1168-82. DOI: 10.1089/neu.2004.21.1168. View

Brockmann R, Beyer A, Heinisch J, Wilhelm T . Posttranscriptional expression regulation: what determines translation rates?. PLoS Comput Biol. 2007; 3(3):e57. PMC: 1829480. DOI: 10.1371/journal.pcbi.0030057. View

Palzur E, Zaaroor M, Vlodavsky E, Milman F, Soustiel J . Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties. Brain Res. 2008; 1221:126-33. DOI: 10.1016/j.brainres.2008.04.078. View

Noack H, Lindenau J, Rothe F, Asayama K, Wolf G . Differential expression of superoxide dismutase isoforms in neuronal and glial compartments in the course of excitotoxically mediated neurodegeneration: relation to oxidative and nitrergic stress. Glia. 1998; 23(4):285-97. DOI: 10.1002/(sici)1098-1136(199808)23:4<285::aid-glia1>3.0.co;2-2. View

Droge W . Free radicals in the physiological control of cell function. Physiol Rev. 2002; 82(1):47-95. DOI: 10.1152/physrev.00018.2001. View

Benedetti S, Lamorgese A, Piersantelli M, Pagliarani S, Benvenuti F, Canestrari F . Oxidative stress and antioxidant status in patients undergoing prolonged exposure to hyperbaric oxygen. Clin Biochem. 2004; 37(4):312-7. DOI: 10.1016/j.clinbiochem.2003.12.001. View

. A review of the scientific evidence on the treatment of traumatic brain injuries and strokes with hyperbaric oxygen. Brain Inj. 2003; 17(3):225-36. DOI: 10.1080/0269905021000030814. View

10.

Brkic P, Stojiljkovic M, Jovanovic T, Dacic S, Lavrnja I, Savic D . Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats. Brain Inj. 2012; 26(10):1273-84. DOI: 10.3109/02699052.2012.667593. View

11.

Xiong Y, Shie F, Zhang J, Lee C, Ho Y . Prevention of mitochondrial dysfunction in post-traumatic mouse brain by superoxide dismutase. J Neurochem. 2005; 95(3):732-44. DOI: 10.1111/j.1471-4159.2005.03412.x. View

12.

Roje Z, Roje Z, Eterovic D, Druzijanic N, Petricevic A, Roje T . Influence of adjuvant hyperbaric oxygen therapy on short-term complications during surgical reconstruction of upper and lower extremity war injuries: retrospective cohort study. Croat Med J. 2008; 49(2):224-32. PMC: 2359875. DOI: 10.3325/cmj.2008.2.224. View

13.

Fournier M, Paulson A, Pavelka N, Mosley A, Gaudenz K, Bradford W . Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin. Mol Cell Proteomics. 2009; 9(2):271-84. PMC: 2830839. DOI: 10.1074/mcp.M900415-MCP200. View

14.

Hampson N, Simonson S, Kramer C, Piantadosi C . Central nervous system oxygen toxicity during hyperbaric treatment of patients with carbon monoxide poisoning. Undersea Hyperb Med. 1996; 23(4):215-9. View

15.

Palzur E, Vlodavsky E, Mulla H, Arieli R, Feinsod M, Soustiel J . Hyperbaric oxygen therapy for reduction of secondary brain damage in head injury: an animal model of brain contusion. J Neurotrauma. 2004; 21(1):41-8. DOI: 10.1089/089771504772695931. View

16.

Bitterman H . Bench-to-bedside review: oxygen as a drug. Crit Care. 2009; 13(1):205. PMC: 2688103. DOI: 10.1186/cc7151. View

17.

Kundrotiene J, Wagner A, Liljequist S . Fluoro-Jade and TUNEL staining as useful tools to identify ischemic brain damage following moderate extradural compression of sensorimotor cortex. Acta Neurobiol Exp (Wars). 2004; 64(2):153-62. DOI: 10.55782/ane-2004-1501. View

18.

Lee H, Pancholi N, Esposito L, Previll L, Wang X, Zhu X . Early induction of oxidative stress in mouse model of Alzheimer disease with reduced mitochondrial superoxide dismutase activity. PLoS One. 2012; 7(1):e28033. PMC: 3261865. DOI: 10.1371/journal.pone.0028033. View

19.

Rockswold S, Rockswold G, Defillo A . Hyperbaric oxygen in traumatic brain injury. Neurol Res. 2007; 29(2):162-72. DOI: 10.1179/016164107X181798. View

20.

Wada K, Miyazawa T, Nomura N, Yano A, Tsuzuki N, Nawashiro H . Mn-SOD and Bcl-2 expression after repeated hyperbaric oxygenation. Acta Neurochir Suppl. 2001; 76:285-90. DOI: 10.1007/978-3-7091-6346-7_59. View