Involvement of Placental/umbilical Cord Blood Acid-base Status and Gas Values on the Radiosensitivity of Human Fetal/neonatal Hematopoietic Stem/progenitor Cells

Overview

Journal J Radiat Res

Publisher Oxford University Press

Specialties Environmental Health
Oncology
Radiology

Date 2012 Dec 25

PMID 23263728

Authors

Masaru Yamaguchi

Satoko Ebina

Ikuo Kashiwakura

Affiliations

Soon will be listed here.

Abstract

Arterial cord blood (CB) acid-base status and gas values, such as pH, PCO2, PO2, HCO3(-)and base excess, provide useful information on the fetal and neonatal condition. However, it remains unknown whether these values affect the radiosensitivity of fetal/neonatal hematopoiesis. The present study evaluated the relationship between arterial CB acid-base status, gas values, and the radiosensitivity of CB hematopoietic stem/progenitor cells (HSPCs). A total of 25 CB units were collected. The arterial CB acid-base status and gas values were measured within 30 min of delivery. The CD34(+)HSPCs obtained from CB were exposed to 2 Gy X-irradiation, and then assayed for colony-forming unit-granulocyte-macrophage, burst-forming unit-erythroid (BFU-E), and colony-forming unit-granulocyte erythroid, macrophage and megakaryocyte cells. Acid-base status and gas values for PCO2and HCO3(-)showed a statistically significant negative correlation with the surviving fraction of BFU-E. In addition, a significant positive correlation was observed between gestational age and PCO2. Moreover, the surviving fraction of BFU-E showed a significant negative correlation with gestational age. Thus, HSPCs obtained from CB with high PCO2/HCO3(-)levels were sensitive to X-irradiation, which suggests that the status of arterial PCO2/HCO3(-)influences the radiosensitivity of fetal/neonatal hematopoiesis, especially erythropoiesis.

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