Production, Characterization, and Species Specificity of Monoclonal Antibodies to Mycobacterium Avium Complex Protein Antigens

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1990 May 1

PMID 2323822

Citations 14

Authors

D A Rouse

S L Morris

A B Karpas

P G Probst

S D Chaparas

Affiliations

Soon will be listed here.

Abstract

The incidence of Mycobacterium avium-Mycobacterium intracellulare complex infections has increased in recent years primarily because a significant proportion of acquired immunodeficiency syndrome patients develop disseminated M. avium complex disease. In an effort to develop new tools to study these infections, we have produced eight monoclonal antibodies directed against M. avium. Western blot (immunoblot) specificity analysis and protease sensitivity assays indicate that four of these antibodies recognize M. avium-specific protein epitopes and two react with M. avium complex-specific peptide determinants. These monoclonal antibodies may be useful clinically in the diagnosis of M. avium complex disease and in the laboratory for isolation and characterization of native and recombinant M. avium complex antigens.

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References

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

Ivanyi J, Sharp K, Jackett P, Bothamley G . Immunological study of the defined constituents of mycobacteria. Springer Semin Immunopathol. 1988; 10(4):279-300. DOI: 10.1007/BF02053841. View

Macher A, Kovacs J, Gill V, Roberts G, Ames J, Park C . Bacteremia due to Mycobacterium avium-intracellulare in the acquired immunodeficiency syndrome. Ann Intern Med. 1983; 99(6):782-5. DOI: 10.7326/0003-4819-99-6-782. View

Hawkins C, Gold J, Whimbey E, Kiehn T, Brannon P, Cammarata R . Mycobacterium avium complex infections in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1986; 105(2):184-8. DOI: 10.7326/0003-4819-105-2-184. View

Kadival G, Chaparas S . Production, characterization, and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis. J Clin Microbiol. 1987; 25(1):76-80. PMC: 265827. DOI: 10.1128/jcm.25.1.76-80.1987. View

Young L, Inderlied C, Berlin O, Gottlieb M . Mycobacterial infections in AIDS patients, with an emphasis on the Mycobacterium avium complex. Rev Infect Dis. 1986; 8(6):1024-33. DOI: 10.1093/clinids/8.6.1024. View

Nishimori K, Yugi H, Naiki M, Sugimura T, Tanaka Y, Nonomura I . Production and characterization of serovar-specific monoclonal antibodies to serovars 4, 8, and 9 of Mycobacterium intracellulare. Infect Immun. 1987; 55(3):711-5. PMC: 260398. DOI: 10.1128/iai.55.3.711-715.1987. View

Shinnick T . The 65-kilodalton antigen of Mycobacterium tuberculosis. J Bacteriol. 1987; 169(3):1080-8. PMC: 211903. DOI: 10.1128/jb.169.3.1080-1088.1987. View

Husson R, Young R . Genes for the major protein antigens of Mycobacterium tuberculosis: the etiologic agents of tuberculosis and leprosy share an immunodominant antigen. Proc Natl Acad Sci U S A. 1987; 84(6):1679-83. PMC: 304500. DOI: 10.1073/pnas.84.6.1679. View

10.

Lu M, Lien M, Becker R, Heine H, Buggs A, Lipovsek D . Genes for immunodominant protein antigens are highly homologous in Mycobacterium tuberculosis, Mycobacterium africanum, and the vaccine strain Mycobacterium bovis BCG. Infect Immun. 1987; 55(10):2378-82. PMC: 260716. DOI: 10.1128/iai.55.10.2378-2382.1987. View

11.

Kadival G, Chaparas S, Hussong D . Characterization of serologic and cell-mediated reactivity of a 38-kDa antigen isolated from Mycobacterium tuberculosis. J Immunol. 1987; 139(7):2447-51. View

12.

Worsaae A, Ljungqvist L, Haslov K, Heron I, Bennedsen J . Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs. Infect Immun. 1987; 55(12):2922-7. PMC: 260007. DOI: 10.1128/iai.55.12.2922-2927.1987. View

13.

Wallace J, Hannah J . Mycobacterium avium complex infection in patients with the acquired immunodeficiency syndrome. A clinicopathologic study. Chest. 1988; 93(5):926-32. DOI: 10.1378/chest.93.5.926. View

14.

Young D, Lathigra R, Hendrix R, Sweetser D, Young R . Stress proteins are immune targets in leprosy and tuberculosis. Proc Natl Acad Sci U S A. 1988; 85(12):4267-70. PMC: 280408. DOI: 10.1073/pnas.85.12.4267. View

15.

Morris S, Rouse D, Hussong D, Chaparas S . Isolation and characterization of a recombinant lambda gt11 bacteriophage which expresses an immunoreactive Mycobacterium intracellulare protein in Escherichia coli. Infect Immun. 1988; 56(12):3026-31. PMC: 259695. DOI: 10.1128/iai.56.12.3026-3031.1988. View

16.

Selwyn P, Hartel D, Lewis V, Schoenbaum E, Vermund S, Klein R . A prospective study of the risk of tuberculosis among intravenous drug users with human immunodeficiency virus infection. N Engl J Med. 1989; 320(9):545-50. DOI: 10.1056/NEJM198903023200901. View

17.

Collins F . AIDS-related mycobacterial disease. Springer Semin Immunopathol. 1988; 10(4):375-91. DOI: 10.1007/BF02053847. View

18.

Abe C, Saito H, Tomioka H, Fukasawa Y . Production of a monoclonal antibody specific for Mycobacterium avium and immunological activity of the affinity-purified antigen. Infect Immun. 1989; 57(4):1095-9. PMC: 313235. DOI: 10.1128/iai.57.4.1095-1099.1989. View

19.

Kolk A, Evers R, Groothuis D, Gilis H, Kuijper S . Production and characterization of monoclonal antibodies against specific serotypes of Mycobacterium avium and the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex. Infect Immun. 1989; 57(8):2514-21. PMC: 313479. DOI: 10.1128/iai.57.8.2514-2521.1989. View

20.

Morris S, Rouse D, Hussong D, Chaparas S . Isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different Mycobacterium intracellulare antigens. Infect Immun. 1990; 58(1):17-20. PMC: 258401. DOI: 10.1128/iai.58.1.17-20.1990. View