Overexpression of Poplar Wounding-inducible Genes in Arabidopsis Caused Improved Resistance Against Helicoverpa Armigera (Hübner) Larvae

Overview

Journal Breed Sci

Date 2012 Dec 11

PMID 23226090

Authors

Rongfeng Hu

Jiehua Wang

Yan Ji

Yingjin Song

Shaohui Yang

Affiliations

Soon will be listed here.

Abstract

Four highly inducible genes of poplar trees, PtdKTI5, PtdWIN4, PtdPOP3 from hybrid poplar (Populus trichocarpa × P. deltoides) and PtKTI2 from trembling aspen (Populus tremuloides Michx.) have been individually transformed into Arabidopsis thaliana for overexpression. High transcriptional level of each transgene in transgenic Arabidopsis lines was confirmed by RT-PCR analysis. The development, body weight and survivorship of cotton bollworm (Helicoverpa armigera) fed on four types of transgenic Arabidopis plants were evaluated in the laboratory. Our data indicated that these four Populus defense-related genes exhibited various degree of insectital activity on larval and postlarval development of cotton bollworm and may be utilized for herbivore resistance improvement in plant genetic engineering.

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