Sequential Triplex Real-time PCR Assay for Detecting 21 Pneumococcal Capsular Serotypes That Account for a High Global Disease Burden

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2012 Dec 11

PMID 23224094

Citations 87

Authors

Fabiana C Pimenta

Alexis Roundtree

Ahmet Soysal

Mustafa Bakir

Mignon du Plessis

Nicole Wolter

Anne von Gottberg

Lesley McGee

Maria da Gloria Carvalho

Bernard Beall

Affiliations

Soon will be listed here.

Abstract

We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.

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