Rapid and Sensitive Detection of Acinetobacter Baumannii Using Loop-mediated Isothermal Amplification

Overview

Journal J Microbiol Methods

Specialty Microbiology

Date 2012 Dec 11

PMID 23220188

Citations 12

Authors

Po-Chi Soo

Chun-Chieh Tseng

Siao-Ru Ling

Ming-Li Liou

Chih-Chin Liu

Huei-Jen Chao

Teng-Yi Lin

Kai-Chih Chang

Affiliations

Soon will be listed here.

Abstract

Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.

Citing Articles

A signal-off aptasensor for the determination of Acinetobacter baumannii by using methylene blue as an electrochemical probe.

Abedi R, Raoof J, Mohseni M, Bagheri Hashkavayi A Mikrochim Acta. 2023; 190(8):308.

PMID: 37466698 DOI: 10.1007/s00604-023-05901-0.

Aptamer Conjugated Indium Phosphide Quantum Dots with a Zinc Sulphide Shell as Photoluminescent Labels for .

Ayed Z, Malhotra S, Dobhal G, Goreham R Nanomaterials (Basel). 2021; 11(12).

PMID: 34947666 PMC: 8703687. DOI: 10.3390/nano11123317.

Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Complex and Associated Carbapenem Resistance.

Sharma A, Gaind R Front Mol Biosci. 2021; 8:659256.

PMID: 34250011 PMC: 8260673. DOI: 10.3389/fmolb.2021.659256.

Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.

Obande G, Singh K Infect Drug Resist. 2020; 13:455-483.

PMID: 32104017 PMC: 7024801. DOI: 10.2147/IDR.S217571.

Prevalence and phenotypic pattern of antibiotic resistance of Acinetobacter baumannii isolated from different types of raw meat samples in Isfahan, Iran.

Askari N, Momtaz H, Tajbakhsh E Vet Med Sci. 2019; 6(1):147-153.

PMID: 31576672 PMC: 7036315. DOI: 10.1002/vms3.199.