MicroRNA-independent Recruitment of Argonaute 1 to Nanos MRNA Through the Smaug RNA-binding Protein

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2012 Nov 28

PMID 23184089

Citations 35

Authors

Benjamin D Pinder

Craig A Smibert

Affiliations

Soon will be listed here.

Abstract

Argonaute (Ago) proteins are typically recruited to target messenger RNAs via an associated small RNA such as a microRNA (miRNA). Here, we describe a new mechanism of Ago recruitment through the Drosophila Smaug RNA-binding protein. We show that Smaug interacts with the Ago1 protein, and that Ago1 interacts with and is required for the translational repression of the Smaug target, nanos mRNA. The Ago1/nanos mRNA interaction does not require a miRNA, but it does require Smaug. Taken together, our data suggest a model whereby Smaug directly recruits Ago1 to nanos mRNA in a miRNA-independent manner, thereby repressing translation.

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