HLA-DQ-controlled T Cell Response to Soluble Egg Antigen of Schistosoma Japonicum in Humans

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1990 Mar 1

PMID 2317945

Citations 2

Authors

N Ohta

T Edahiro

A Ishii

M Yasukawa

Y Hosaka

Affiliations

Soon will be listed here.

Abstract

We analysed regulatory mechanisms of the human T cell response to soluble egg antigen (SEA) of Schistosoma japonicum in vitro. SEA is a crude antigen mixture containing numerous epitopes. We obtained SEA-induced T cell lines from five patients with chronic schistosomiasis japonica, and tested their proliferative response to molecular weight fractions of SEA. Although all T cell lines showed strong responses to crude SEA, there was a heterogeneity in fraction-driven responsiveness. All but one T cell line tested failed to respond to SEA fraction I (mol. wt greater than 18 kD). One patient who was typed as HLA-DQw1/w4, did not show proliferation of CD4+ T cells to fraction I; however, a fraction I-driven helper T cell response was observed when we added HU-11 monoclonal antibody specific for HLA-DQw1/w4. This indicated that the patient had helper T cells to the fraction even though their response was suppressed. Because HLA-DQ had an effect on functional expression of suppressor T cells, it was suggested that there was epitope-specific regulation of the T cell response to SEA, and HLA-DQ-controlled immune suppression might be involved in the regulatory system in human chronic schistosome infection.

Citing Articles

Lymphoproliferative responses to a merozoite surface antigen of Plasmodium falciparum: preliminary evidence for seasonal activation of CD8+/HLA-DQ-restricted suppressor cells.

Riley E, Morris-Jones S, Taylor-Robinson A, Holder A Clin Exp Immunol. 1993; 94(1):64-7.

PMID: 8403519 PMC: 1534374. DOI: 10.1111/j.1365-2249.1993.tb05978.x.

Regulation of the human T-cell response to Schistosoma japonicum egg antigen by concomitant cellular and humoral mechanisms in vitro.

Ohta N, Asahi H, Hosaka Y, Minai M, Ishii A Parasitol Res. 1991; 77(1):54-8.

PMID: 1899724 DOI: 10.1007/BF00934386.

References

CHEEVER A, Byram J, VON LICHTENBERG F . Immunopathology of Schistosoma japonicum infection in athymic mice. Parasite Immunol. 1985; 7(4):387-98. DOI: 10.1111/j.1365-3024.1985.tb00085.x. View

Kasahara M, Takenouchi T, Ikeda H, Ogasawara K, Okuyama T, Ishikawa N . Serologic dissection of HLA-D specificities by the use of monoclonal antibodies. II. Distinction between HLA-Dw2 and HLA-Dw12. Immunogenetics. 1983; 18(5):525-36. DOI: 10.1007/BF00364393. View

Sidner R, CARTER C, Colley D . Modulation of Schistosoma japonicum pulmonary egg granulomas with monoclonal antibodies. Am J Trop Med Hyg. 1987; 36(2):361-70. DOI: 10.4269/ajtmh.1987.36.361. View

Tweardy D, Osman G, El Kholy A, Ellner J . Failure of immunosuppressive mechanisms in human Schistosoma mansoni infection with hepatosplenomegaly. J Clin Microbiol. 1987; 25(5):768-73. PMC: 266086. DOI: 10.1128/jcm.25.5.768-773.1987. View

Hirayama K, Matsushita S, Kikuchi I, Iuchi M, Ohta N, Sasazuki T . HLA-DQ is epistatic to HLA-DR in controlling the immune response to schistosomal antigen in humans. Nature. 1987; 327(6121):426-30. DOI: 10.1038/327426a0. View

Yasukawa M, Iwamasa K, Kawamura S, Murakami S, Takada K, Hato T . Phenotypic and genotypic analysis of chronic myelogenous leukaemia with T lymphoblastic and megakaryoblastic mixed crisis. Br J Haematol. 1987; 66(3):331-6. DOI: 10.1111/j.1365-2141.1987.tb06919.x. View

Ragheb S, Mathew R, Boros D . Establishment and characterization of an antigen-specific T-cell line from liver granulomas of Schistosoma mansoni-infected mice. Infect Immun. 1987; 55(11):2625-30. PMC: 259952. DOI: 10.1128/iai.55.11.2625-2630.1987. View

Auriault C, Gras-Masse H, Wolowczuk I, Pierce R, Balloul J, Neyrinck J . Analysis of T and B cell epitopes of the Schistosoma mansoni P28 antigen in the rat model by using synthetic peptides. J Immunol. 1988; 141(5):1687-94. View

Ohta N, Edahiro T, Tohgi N, Ishii A, Minai M, Hosaka Y . Generation and functional characterization of T cell lines and clones specific for Schistosoma japonicum egg antigen in humans. J Immunol. 1988; 141(7):2445-50. View

10.

Harn D, Danko K, Quinn J, Stadecker M . Schistosoma mansoni: the host immune response to egg antigens. I. Partial characterization of cellular and humoral responses to pI fractions of soluble egg antigens. J Immunol. 1989; 142(6):2061-6. View

11.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

12.

Boros D, Pelley R, Warren K . Spontaneous modulation of granulomatous hypersensitivity in schistosomiasis mansoni. J Immunol. 1975; 114(5):1437-41. View

13.

Byram J, Doenhoff M, Musallam R, Brink L, VON LICHTENBERG F . Schistosoma mansoni infections in T-cell deprived mice, and the ameliorating effect of administering homologous chronic infection serum. II. Pathology. Am J Trop Med Hyg. 1979; 28(2):274-85. DOI: 10.4269/ajtmh.1979.28.274. View

14.

Sasazuki T, Ohta N, Kaneoka R, Kojima S . Association between an HLA haplotype and low responsiveness to schistosomal worm antigen in man. J Exp Med. 1980; 152(2 Pt 2):314s-318s. View

15.

Colley D . T lymphocytes that contribute to the immunoregulation of granuloma formation in chronic murine schistosomiasis. J Immunol. 1981; 126(4):1465-8. View

16.

Olds G, Olveda R, Tracy J, Mahmoud A . Adoptive transfer of modulation of granuloma formation and hepatosplenic disease in murine schistosomiasis japonica by serum from chronically infected animals. J Immunol. 1982; 128(3):1391-3. View

17.

Ishii A, Owhashi M, Hashiguchi J . Schistosoma japonicum: immediate hypersensitivity reactions to egg antigen in humans and rodents. Exp Parasitol. 1982; 54(1):104-12. DOI: 10.1016/0014-4894(82)90116-3. View

18.

Koide Y, Awashima F, YOSHIDA T, Takenouchi T, Wakisaka A, Moriuchi J . The role of three distinct Ia-like antigen molecules in human T cell proliferative responses: effect of monoclonal anti-Ia-like antibodies. J Immunol. 1982; 129(3):1061-9. View

19.

Ohta N, Nishimura Y, Iuchi M, Sasazuki T . Immunogenetic analysis of patients with post-schistosomal liver cirrhosis in man. Clin Exp Immunol. 1982; 49(3):493-9. PMC: 1536731. View

20.

Ohta N, Minai M, Sasazuki T . Antigen-specific suppressor T lymphocytes (Leu-2a+3a-) in human schistosomiasis japonica. J Immunol. 1983; 131(5):2524-8. View