Arg188 in Rice Sucrose Transporter OsSUT1 is Crucial for Substrate Transport

Overview

Journal BMC Biochem

Publisher Biomed Central

Specialty Biochemistry

Date 2012 Nov 23

PMID 23170937

Citations 6

Authors

Ye Sun

John M Ward

Affiliations

Soon will be listed here.

Abstract

Background: Plant sucrose uptake transporters (SUTs) are H+/sucrose symporters related to the major facilitator superfamily (MFS). SUTs are essential for plant growth but little is known about their transport mechanism. Recent work identified several conserved, charged amino acids within transmembrane spans (TMS) in SUTs that are essential for transport activity. Here we further evaluated the role of one of these positions, R188 in the fourth TMS of OsSUT1, a type II SUT.

Results: The OsSUT1(R188K) mutant, studied by expression in plants, yeast, and Xenopus oocytes, did not transport sucrose but showed a H+ leak that was blocked by sucrose. The H+ leak was also blocked by β-phenyl glucoside which is not translocated by OsSUT1. Replacing the corresponding Arg in type I and type III SUTs, AtSUC1(R163K) and LjSUT4(R169K), respectively, also resulted in loss of sucrose transport activity. Fluorination at the glucosyl 3 and 4 positions of α-phenyl glucoside greatly decreased transport by wild type OsSUT1 but did not affect the ability to block H+ leak in the R188K mutant.

Conclusion: OsSUT1 R188 appears to be essential for sucrose translocation but not for substrate interaction that blocks H+ leak. Therefore, we propose that an additional binding site functions in the initial recognition of substrates. The corresponding Arg in type I and III SUTs are equally important. We propose that R188 interacts with glucosyl 3-OH and 4-OH during translocation.

Citing Articles

Structure and sucrose binding mechanism of the plant SUC1 sucrose transporter.

Bavnhoj L, Driller J, Zuzic L, Stange A, Schiott B, Pedersen B Nat Plants. 2023; 9(6):938-950.

PMID: 37188854 PMC: 10281868. DOI: 10.1038/s41477-023-01421-0.

Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola-caused giant cells in rice.

Xu L, Xiao L, Xiao Y, Peng D, Xiao X, Huang W Mol Plant Pathol. 2021; 22(5):539-550.

PMID: 33723908 PMC: 8035636. DOI: 10.1111/mpp.13042.

SUT1.1 is a high affinity sucrose-proton co-transporter.

Santiago J, Ward J, Sharkey T Plant Direct. 2020; 4(8):e00260.

PMID: 32885136 PMC: 7453976. DOI: 10.1002/pld3.260.

Expression of Sucrose Transporters from Confer High Yield and Enhances Drought Resistance in Arabidopsis.

Cai Y, Yan J, Tu W, Deng Z, Dong W, Gao H Int J Mol Sci. 2020; 21(7).

PMID: 32283825 PMC: 7177370. DOI: 10.3390/ijms21072624.

Probing binding specificity of the sucrose transporter AtSUC2 with fluorescent coumarin glucosides.

de Moliner F, Knox K, Reinders A, Ward J, McLaughlin P, Oparka K J Exp Bot. 2018; 69(10):2473-2482.

PMID: 29506213 PMC: 5920547. DOI: 10.1093/jxb/ery075.

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