Complete Genome of a Novel Porcine Astrovirus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2012 Nov 21

PMID 23166236

Citations 9

Authors

Tongling Shan

Chunmei Wang

Wu Tong

Hao Zheng

Xiuguo Hua

Shen Yang

Yifei Guo

Wen Zhang

Guangzhi Tong

Affiliations

Soon will be listed here.

Abstract

Astroviruses have been widely described in mammalian and avian species. Here, we report a complete genome sequence of a novel porcine astrovirus (PoAstV) isolated from a porcine fecal sample in China. The genome consists of 6,611 nucleotides, excluding the 3' poly(A) tail, and has two open reading frames (ORFs). ORF1 maps between nucleotide positions 19 and 4211 and encodes a 1,396-amino-acid (aa) polyprotein precursor consisting of nonstructural protein and putative RNA-dependent RNA polymerase, and ORF2 maps between nucleotide positions 4202 and 6531 and encodes a 775-aa polyprotein which is a capsid precursor protein. The genome sequence of the virus was distinct enough from those of the known PoAstVs to be considered a novel sequence. Phylogenetic analysis based on the predicted amino acid sequence of the complete capsid region showed that this strain may be a novel porcine astrovirus.

Citing Articles

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References

Harbour D, Ashley C, Williams P, Gruffydd-Jones T . Natural and experimental astrovirus infection of cats. Vet Rec. 1987; 120(23):555-7. DOI: 10.1136/vr.120.23.555. View

Kapoor A, Li L, Victoria J, Oderinde B, Mason C, Pandey P . Multiple novel astrovirus species in human stool. J Gen Virol. 2009; 90(Pt 12):2965-2972. PMC: 2796194. DOI: 10.1099/vir.0.014449-0. View

Koci M, Seal B, Schultz-Cherry S . Molecular characterization of an avian astrovirus. J Virol. 2000; 74(13):6173-7. PMC: 112117. DOI: 10.1128/jvi.74.13.6173-6177.2000. View

Zhao W, Zhu A, Yuan C, Yu Y, Zhu C, Lan D . Detection of astrovirus infection in pigeons (Columbia livia) during an outbreak of diarrhoea. Avian Pathol. 2011; 40(4):361-5. DOI: 10.1080/03079457.2011.587792. View

Lan D, Ji W, Shan T, Cui L, Yang Z, Yuan C . Molecular characterization of a porcine astrovirus strain in China. Arch Virol. 2011; 156(10):1869-75. PMC: 7086730. DOI: 10.1007/s00705-011-1050-8. View

Indik S, Valicek L, Smid B, Dvorakova H, Rodak L . Isolation and partial characterization of a novel porcine astrovirus. Vet Microbiol. 2006; 117(2-4):276-83. DOI: 10.1016/j.vetmic.2006.06.020. View

Atkins A, Wellehan Jr J, Childress A, Archer L, Fraser W, Citino S . Characterization of an outbreak of astroviral diarrhea in a group of cheetahs (Acinonyx jubatus). Vet Microbiol. 2009; 136(1-2):160-5. PMC: 7117330. DOI: 10.1016/j.vetmic.2008.10.035. View

Shan T, Lan D, Li L, Wang C, Cui L, Zhang W . Genomic characterization and high prevalence of bocaviruses in swine. PLoS One. 2011; 6(4):e17292. PMC: 3078135. DOI: 10.1371/journal.pone.0017292. View

Rivera R, Nollens H, Venn-Watson S, Gulland F, Wellehan Jr J . Characterization of phylogenetically diverse astroviruses of marine mammals. J Gen Virol. 2009; 91(Pt 1):166-73. DOI: 10.1099/vir.0.015222-0. View

10.

Reynolds D, Saif Y . Astrovirus: a cause of an enteric disease in turkey poults. Avian Dis. 1986; 30(4):728-35. View

11.

Finkbeiner S, Le B, Holtz L, Storch G, Wang D . Detection of newly described astrovirus MLB1 in stool samples from children. Emerg Infect Dis. 2009; 15(3):441-4. PMC: 2666294. DOI: 10.3201/eid1503.081213. View

12.

Woode G, Gourley N, Pohlenz J, Liebler E, Mathews S, HUTCHINSON M . Serotypes of bovine astrovirus. J Clin Microbiol. 1985; 22(4):668-70. PMC: 268492. DOI: 10.1128/jcm.22.4.668-670.1985. View

13.

Kurtz J, Lee T, Pickering D . Astrovirus associated gastroenteritis in a children's ward. J Clin Pathol. 1977; 30(10):948-52. PMC: 476601. DOI: 10.1136/jcp.30.10.948. View

14.

Snodgrass D, Angus K, Gray E, MENZIES J, Paul G . Pathogenesis of diarrhoea caused by astrovirus infections in lambs. Arch Virol. 1979; 60(3-4):217-26. PMC: 7086881. DOI: 10.1007/BF01317493. View

15.

Monroe S, Jiang B, Stine S, Koopmans M, Glass R . Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. J Virol. 1993; 67(6):3611-4. PMC: 237710. DOI: 10.1128/JVI.67.6.3611-3614.1993. View

16.

Tzipori S, MENZIES J, Gray E . Detection of astrovirus in the faeces of red deer. Vet Rec. 1981; 108(13):286. DOI: 10.1136/vr.108.13.286. View

17.

Zhu H, Chu D, Liu W, Dong B, Zhang S, Zhang J . Detection of diverse astroviruses from bats in China. J Gen Virol. 2009; 90(Pt 4):883-887. DOI: 10.1099/vir.0.007732-0. View

18.

Li L, Shan T, Wang C, Cote C, Kolman J, Onions D . The fecal viral flora of California sea lions. J Virol. 2011; 85(19):9909-17. PMC: 3196430. DOI: 10.1128/JVI.05026-11. View

19.

Toffan A, Jonassen C, De Battisti C, Schiavon E, Kofstad T, Capua I . Genetic characterization of a new astrovirus detected in dogs suffering from diarrhoea. Vet Microbiol. 2009; 139(1-2):147-52. PMC: 7126621. DOI: 10.1016/j.vetmic.2009.04.031. View

20.

Reuter G, Pankovics P, Boros A . Identification of a novel astrovirus in a domestic pig in Hungary. Arch Virol. 2010; 156(1):125-8. PMC: 7086753. DOI: 10.1007/s00705-010-0827-5. View