Goniometric Measurements of Thick Tissue Using Monte Carlo Simulations to Obtain the Single Scattering Anisotropy Coefficient

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2012 Nov 20

PMID 23162710

Citations 19

Authors

Gunnsteinn Hall

Steven L Jacques

Kevin W Eliceiri

Paul J Campagnola

Affiliations

Soon will be listed here.

Abstract

The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.

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