MicroRNA-195 Suppresses Osteosarcoma Cell Invasion and Migration in Vitro by Targeting FASN

Overview

Journal Oncol Lett

Specialty Oncology

Date 2012 Nov 20

PMID 23162665

Citations 44

Authors

Jian Hua Mao

Rong Ping Zhou

Ai Fen Peng

Zhi Li Liu

Shan Hu Huang

Xin Hua Long

Yong Shu

Affiliations

Soon will be listed here.

Abstract

microRNAs are involved in different cancer-related processes. miR-195, one of the miR-16/15/195/424/497 family members, has been shown to act as a tumor suppressor during tumorigenesis. However, the function of miR-195 in osteosarcoma is still unclear. In our study, the miR-195 expression level was upregulated in osteosarcoma cells, by transfection with miR-195, and the fatty acid synthase (FASN) mRNA and protein expression levels were measured by RT-PCR and western blotting. Cell migration and invasion was measured using wound healing migration and Transwell invasion assays. We found that the upregulation of miR-195 greatly decreased cell invasion and the migration of U2OS. We also identified that FASN may be a direct target of miR-195 by the luciferase activity assay. These findings provide evidence that miR-195 plays a key role in inhibiting osteosarcoma cell migration and invasion through targeting FASN, and strongly suggest that exogenous miR-195 may have therapeutic value in treating osteosarcoma.

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