The Requirement for Carotenoids in the Assembly and Function of the Photosynthetic Complexes in Chlamydomonas Reinhardtii

Overview

Journal Plant Physiol

Specialty Physiology

Date 2012 Nov 20

PMID 23161889

Citations 19

Authors

Stefano Santabarbara

Anna Paola Casazza

Kulsam Ali

Chloe K Economou

Thanyanun Wannathong

Francesca Zito

Kevin E Redding

Fabrice Rappaport

Saul Purton

Affiliations

Soon will be listed here.

Abstract

We have investigated the importance of carotenoids on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids. The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid biosynthesis pathway, and therefore is unable to synthesize any carotenes and xanthophylls. We find that FN68 is unable to accumulate the light-harvesting complexes associated with both photosystems as well as the RC subunits of photosystem II. The accumulation of the cytochrome b₆f complex is also strongly reduced to a level approximately 10% that of the wild type. However, the residual fraction of assembled cytochrome b₆f complexes exhibits single-turnover electron transfer kinetics comparable to those observed in the wild-type strain. Surprisingly, photosystem I is assembled to significant levels in the absence of carotenoids in FN68 and possesses functional properties that are very similar to those of the wild-type complex.

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