Laminar Silk Scaffolds for Aligned Tissue Fabrication

Overview

Journal Macromol Biosci

Specialties Biochemistry
Biology

Date 2012 Nov 20

PMID 23161731

Citations 8

Authors

Biman B Mandal

Eun Seok Gil

Bruce Panilaitis

David L Kaplan

Affiliations

Soon will be listed here.

Abstract

3D-biomaterial scaffolds with aligned architecture are of vital importance in tissue regeneration. A generic method is demonstrated to produce aligned biomaterial scaffolds using the physics of directional ice freezing. Homogeneously aligned 3D silk scaffolds with high porosity and alignment are prepared. The method can be adapted to a wide range of polymers and is devoid of any chemical reactions, thus avoiding potential complications associated with by-products. Mechanical properties and cellular responses with chondrocytes and bone-marrow-derived hMSCs are studied, assessing survival, proliferation, and differentiation. In vivo tests suggest biocompatibility of the matrices for future tissue engineering applications, specifically in areas where high cellular alignment is needed.

Citing Articles

Developing Porous Fibrin Scaffolds with Tunable Anisotropic Features to Direct Myoblast Orientation.

Samolyk B, Pace Z, Li J, Billiar K, Coburn J, Whittington C Tissue Eng Part C Methods. 2024; 30(5):217-228.

PMID: 38562112 PMC: 11812604. DOI: 10.1089/ten.TEC.2023.0363.

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.

Bomkamp C, Skaalure S, Fernando G, Ben-Arye T, Swartz E, Specht E Adv Sci (Weinh). 2021; 9(3):e2102908.

PMID: 34786874 PMC: 8787436. DOI: 10.1002/advs.202102908.

Estimating Kinetic Rate Parameters for Enzymatic Degradation of Lyophilized Silk Fibroin Sponges.

Jameson J, Pacheco M, Butler J, Stoppel W Front Bioeng Biotechnol. 2021; 9:664306.

PMID: 34295878 PMC: 8290342. DOI: 10.3389/fbioe.2021.664306.

Bi-layered Tubular Microfiber Scaffolds as Functional Templates for Engineering Human Intestinal Smooth Muscle Tissue.

Chen Y, Guo C, Manousiouthakis E, Wang X, Cairns D, Roh T Adv Funct Mater. 2021; 30(17).

PMID: 33692658 PMC: 7938961. DOI: 10.1002/adfm.202000543.

Silk-based multilayered angle-ply annulus fibrosus construct to recapitulate form and function of the intervertebral disc.

Bhunia B, Kaplan D, Mandal B Proc Natl Acad Sci U S A. 2017; 115(3):477-482.

PMID: 29282316 PMC: 5776988. DOI: 10.1073/pnas.1715912115.

References

Altman G, Diaz F, Jakuba C, Calabro T, Horan R, Chen J . Silk-based biomaterials. Biomaterials. 2002; 24(3):401-16. DOI: 10.1016/s0142-9612(02)00353-8. View

Dal Pra I, Freddi G, Minic J, Chiarini A, Armato U . De novo engineering of reticular connective tissue in vivo by silk fibroin nonwoven materials. Biomaterials. 2004; 26(14):1987-99. DOI: 10.1016/j.biomaterials.2004.06.036. View

Mooney D, Baldwin D, Suh N, Vacanti J, Langer R . Novel approach to fabricate porous sponges of poly(D,L-lactic-co-glycolic acid) without the use of organic solvents. Biomaterials. 1996; 17(14):1417-22. DOI: 10.1016/0142-9612(96)87284-x. View

Mandal B, Park S, Gil E, Kaplan D . Stem cell-based meniscus tissue engineering. Tissue Eng Part A. 2011; 17(21-22):2749-61. PMC: 3204205. DOI: 10.1089/ten.TEA.2011.0031. View

Wang Y, Blasioli D, Kim H, Kim H, Kaplan D . Cartilage tissue engineering with silk scaffolds and human articular chondrocytes. Biomaterials. 2006; 27(25):4434-42. DOI: 10.1016/j.biomaterials.2006.03.050. View

Wang Y, Rudym D, Walsh A, Abrahamsen L, Kim H, Kim H . In vivo degradation of three-dimensional silk fibroin scaffolds. Biomaterials. 2008; 29(24-25):3415-28. PMC: 3206261. DOI: 10.1016/j.biomaterials.2008.05.002. View

Zhang H, Hussain I, Brust M, Butler M, Rannard S, Cooper A . Aligned two- and three-dimensional structures by directional freezing of polymers and nanoparticles. Nat Mater. 2005; 4(10):787-93. DOI: 10.1038/nmat1487. View

Mandal B, Kundu S . Osteogenic and adipogenic differentiation of rat bone marrow cells on non-mulberry and mulberry silk gland fibroin 3D scaffolds. Biomaterials. 2009; 30(28):5019-30. DOI: 10.1016/j.biomaterials.2009.05.064. View

Santin M, Motta A, Freddi G, Cannas M . In vitro evaluation of the inflammatory potential of the silk fibroin. J Biomed Mater Res. 1999; 46(3):382-9. DOI: 10.1002/(sici)1097-4636(19990905)46:3<382::aid-jbm11>3.0.co;2-r. View

10.

Bhardwaj N, Kundu S . Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv. 2010; 28(3):325-47. DOI: 10.1016/j.biotechadv.2010.01.004. View

11.

Kenar H, Kose G, Toner M, Kaplan D, Hasirci V . A 3D aligned microfibrous myocardial tissue construct cultured under transient perfusion. Biomaterials. 2011; 32(23):5320-9. PMC: 4492442. DOI: 10.1016/j.biomaterials.2011.04.025. View

12.

Omenetto F, Kaplan D . New opportunities for an ancient material. Science. 2010; 329(5991):528-31. PMC: 3136811. DOI: 10.1126/science.1188936. View

13.

Patist C, Mulder M, Gautier S, Maquet V, Jerome R, Oudega M . Freeze-dried poly(D,L-lactic acid) macroporous guidance scaffolds impregnated with brain-derived neurotrophic factor in the transected adult rat thoracic spinal cord. Biomaterials. 2003; 25(9):1569-82. DOI: 10.1016/s0142-9612(03)00503-9. View

14.

Deville S, Saiz E, Tomsia A . Freeze casting of hydroxyapatite scaffolds for bone tissue engineering. Biomaterials. 2006; 27(32):5480-9. DOI: 10.1016/j.biomaterials.2006.06.028. View

15.

Mukai S, Nishihara H, Tamon H . Formation of monolithic silica gel microhoneycombs (SMHs) using pseudosteady state growth of microstructural ice crystals. Chem Commun (Camb). 2004; (7):874-5. DOI: 10.1039/b316597c. View

16.

Deville S, Saiz E, Nalla R, Tomsia A . Freezing as a path to build complex composites. Science. 2006; 311(5760):515-8. DOI: 10.1126/science.1120937. View

17.

Place E, Evans N, Stevens M . Complexity in biomaterials for tissue engineering. Nat Mater. 2009; 8(6):457-70. DOI: 10.1038/nmat2441. View

18.

Lutolf M, Weber F, Schmoekel H, Schense J, Kohler T, Muller R . Repair of bone defects using synthetic mimetics of collagenous extracellular matrices. Nat Biotechnol. 2003; 21(5):513-8. DOI: 10.1038/nbt818. View

19.

Sherwood J, Riley S, Palazzolo R, Brown S, Monkhouse D, Coates M . A three-dimensional osteochondral composite scaffold for articular cartilage repair. Biomaterials. 2002; 23(24):4739-51. DOI: 10.1016/s0142-9612(02)00223-5. View

20.

Mandal B, Kundu S . Cell proliferation and migration in silk fibroin 3D scaffolds. Biomaterials. 2009; 30(15):2956-65. DOI: 10.1016/j.biomaterials.2009.02.006. View