Nicotinamide Mononucleotide Adenylyltransferase Maintains Active Zone Structure by Stabilizing Bruchpilot

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2012 Nov 17

PMID 23154466

Citations 16

Authors

Shaoyun Zang

Yousuf O Ali

Kai Ruan

R Grace Zhai

Affiliations

Soon will be listed here.

Abstract

Active zones are specialized presynaptic structures critical for neurotransmission. We show that a neuronal maintenance factor, nicotinamide mononucleotide adenylyltransferase (NMNAT), is required for maintaining active zone structural integrity in Drosophila by interacting with the active zone protein, Bruchpilot (BRP), and shielding it from activity-induced ubiquitin-proteasome-mediated degradation. NMNAT localizes to the peri-active zone and interacts biochemically with BRP in an activity-dependent manner. Loss of NMNAT results in ubiquitination, mislocalization and aggregation of BRP, and subsequent active zone degeneration. We propose that, as a neuronal maintenance factor, NMNAT specifically maintains active zone structure by direct protein-protein interaction.

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