Characterization of Three Newly Established Rat Sarcoma Cell Clones

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 2012 Nov 15

PMID 23150138

Citations 2

Authors

Monika Holubova

Martin Leba

Marketa Sedmikova

Luca Vannucci

Vratislav Horak

Affiliations

Soon will be listed here.

Abstract

Establishment of new animal models using selected cell lines with different behaviour is very important for cancer investigations. In this study, we describe three morphologically distinct rat sarcoma clones-C4, C7 and D6-isolated from the R5-28 cell line. Cells of all clones expressed vimentin, fibronectin, laminin, collagen IV and matrix metalloproteinases 2 and 9. However, desmin, cytokeratins 8 and 18, ZO-1 and desmoplakins I and II were not detected. Significant proliferative capacity was documented by proliferating cell nuclear antigen expression and BrdU positivity. Karyotype of the C4, C7 and D6 cells greatly differed from diploid chromosome number of normal rat somatic cells. High expression of three cytokines-monocyte chemoattractant protein 1, tissue inhibitor of metalloproteinases 1 and vascular endothelial growth factor-was observed in all three clones. However, they varied in concentration of chemokines associated with neutrophil migration and activation-cytokine induced neutrophil chemoattractant 2 and lipopolysaccharide induced CXC chemokine. The C4 clone showed spontaneous tumour regression in vivo that was associated with significant changes in lymphocyte subpopulations.

Citing Articles

Complex Interplay of Genes Underlies Invasiveness in Fibrosarcoma Progression Model.

Kripnerova M, Parmar H, Sana J, Kopkova A, Radova L, Sopper S J Clin Med. 2021; 10(11).

PMID: 34070472 PMC: 8197499. DOI: 10.3390/jcm10112297.

Immunohistochemical Evidence of the Involvement of Natural Killer (CD161) Cells in Spontaneous Regression of Lewis Rat Sarcoma.

Kovalska J, Cervinkova M, Chmelikova E, Planska D, cizkova J, Horak V In Vivo. 2018; 33(1):47-52.

PMID: 30587601 PMC: 6364084. DOI: 10.21873/invivo.11437.

References

Strieter R, Wiggins R, Phan S, Wharram B, Showell H, Remick D . Monocyte chemotactic protein gene expression by cytokine-treated human fibroblasts and endothelial cells. Biochem Biophys Res Commun. 1989; 162(2):694-700. DOI: 10.1016/0006-291x(89)92366-8. View

Pokorna E, Zicha D, Chaloupkova A, Matouskova E, Vesely P . Two dynamic morphotypes of sarcoma cells, asymmetric stellate and triangle with leading lamella, are related to malignancy. Folia Biol (Praha). 2003; 49(1):33-9. View

Thomas J, Badini M . The role of innate immunity in spontaneous regression of cancer. Indian J Cancer. 2011; 48(2):246-51. DOI: 10.4103/0019-509X.82887. View

Akahane T, Akahane M, Shah A, Connor C, Thorgeirsson U . TIMP-1 inhibits microvascular endothelial cell migration by MMP-dependent and MMP-independent mechanisms. Exp Cell Res. 2004; 301(2):158-67. DOI: 10.1016/j.yexcr.2004.08.002. View

Barnetson R, Halliday G . Regression in skin tumours: a common phenomenon. Australas J Dermatol. 1997; 38 Suppl 1:S63-5. DOI: 10.1111/j.1440-0960.1997.tb01013.x. View

Mackall C, Meltzer P, Helman L . Focus on sarcomas. Cancer Cell. 2002; 2(3):175-8. DOI: 10.1016/s1535-6108(02)00132-0. View

Dodd R, Mito J, Kirsch D . Animal models of soft-tissue sarcoma. Dis Model Mech. 2010; 3(9-10):557-66. PMC: 2931534. DOI: 10.1242/dmm.005223. View

Leader M, Collins M, Patel J, Henry K . Vimentin: an evaluation of its role as a tumour marker. Histopathology. 1987; 11(1):63-72. DOI: 10.1111/j.1365-2559.1987.tb02609.x. View

Kalialis L, Drzewiecki K, Klyver H . Spontaneous regression of metastases from melanoma: review of the literature. Melanoma Res. 2009; 19(5):275-82. DOI: 10.1097/CMR.0b013e32832eabd5. View

10.

Taketo M . Mouse models of gastrointestinal tumors. Cancer Sci. 2006; 97(5):355-61. PMC: 11158113. DOI: 10.1111/j.1349-7006.2006.00190.x. View

11.

Maquoi E, Frankenne F, Noel A, Krell H, Grams F, Foidart J . Type IV collagen induces matrix metalloproteinase 2 activation in HT1080 fibrosarcoma cells. Exp Cell Res. 2000; 261(2):348-59. DOI: 10.1006/excr.2000.5063. View

12.

Horak V, Flechon J . Immunocytochemical characterisation of rabbit and mouse embryonic fibroblasts. Reprod Nutr Dev. 1999; 38(6):683-95. DOI: 10.1051/rnd:19980610. View

13.

Misra A, Mistry N, Grimer R, Peart F . The management of soft tissue sarcoma. J Plast Reconstr Aesthet Surg. 2008; 62(2):161-74. DOI: 10.1016/j.bjps.2008.08.018. View

14.

Balamayooran G, Batra S, Balamayooran T, Cai S, Jeyaseelan S . Monocyte chemoattractant protein 1 regulates pulmonary host defense via neutrophil recruitment during Escherichia coli infection. Infect Immun. 2011; 79(7):2567-77. PMC: 3191985. DOI: 10.1128/IAI.00067-11. View

15.

Tammela T, Enholm B, Alitalo K, Paavonen K . The biology of vascular endothelial growth factors. Cardiovasc Res. 2005; 65(3):550-63. DOI: 10.1016/j.cardiores.2004.12.002. View

16.

Shor A, Keschman E, Lee F, Muro-Cacho C, Letson G, Trent J . Dasatinib inhibits migration and invasion in diverse human sarcoma cell lines and induces apoptosis in bone sarcoma cells dependent on SRC kinase for survival. Cancer Res. 2007; 67(6):2800-8. DOI: 10.1158/0008-5472.CAN-06-3469. View

17.

Penichet M, Dela Cruz J, Rosenblatt J, Morrison S . A murine B cell lymphoma expressing human HER2 / neu undergoes spontaneous tumor regression and elicits antitumor immunity. Cancer Immunol Immunother. 2001; 49(12):649-62. PMC: 11036999. DOI: 10.1007/s002620000155. View

18.

CARR M, Roth S, Luther E, ROSE S, Springer T . Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant. Proc Natl Acad Sci U S A. 1994; 91(9):3652-6. PMC: 43639. DOI: 10.1073/pnas.91.9.3652. View

19.

Jain S, Xu R, Prieto V, Lee P . Molecular classification of soft tissue sarcomas and its clinical applications. Int J Clin Exp Pathol. 2010; 3(4):416-28. PMC: 2872748. View

20.

Mantovani A . The yin-yang of tumor-associated neutrophils. Cancer Cell. 2009; 16(3):173-4. DOI: 10.1016/j.ccr.2009.08.014. View