Detection and Differential Diagnosis of Colon Cancer by a Cumulative Analysis of Promoter Methylation

Overview

Journal Nat Commun

Specialty Biology

Date 2012 Nov 15

PMID 23149750

Citations 17

Authors

Qiong Yang

Ying Dong

Wei Wu

Chunlei Zhu

Hui Chong

Jiangyang Lu

Dehai Yu

Libing Liu

Fengting Lv

Shu Wang

Affiliations

Soon will be listed here.

Abstract

Alterations in the methylation of promoters of cancer-related genes are promising biomarkers for the early detection of disease. Compared with single methylation alteration, assessing combined methylation alterations can provide higher association with specific cancer. Here we use cationic conjugated polymer-based fluorescence resonance energy transfer to quantitatively analyse DNA methylation levels of seven colon cancer-related genes in a Chinese population. Through a stepwise discriminant analysis and cumulative detection of methylation alterations, we acquire high accuracy and sensitivity for colon cancer detection (86.3 and 86.7%) and for differential diagnosis (97.5 and 94%). Moreover, we identify a correlation between the CpG island methylator phenotype and clinically important parameters in patients with colon cancer. The cumulative analysis of promoter methylation alterations by the cationic conjugated polymer-based fluorescence resonance energy transfer may be useful for the screening and differential diagnosis of patients with colon cancer, and for performing clinical correlation analyses.

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