Plasma Cysteine/cystine Reduction Potential Correlates with Plasma Creatinine Levels in Chronic Kidney Disease

Overview

Journal Blood Purif

Specialty Hematology

Date 2012 Nov 14

PMID 23147870

Citations 19

Authors

Silvia D Rodrigues

Giuseppe B Batista

Max Ingberman

Roberto Pecoits-Filho

Lia S Nakao

Affiliations

Soon will be listed here.

Abstract

Background/aims: Oxidative stress has been considered a nontraditional risk factor for cardiovascular disease in the chronic kidney disease (CKD) population, possibly triggered by uremic toxicity.

Methods: A chromatographic method with coulometric detection was adapted to directly and simultaneously determine cysteine (Cys) and cystine (Cyss) in plasma samples. Healthy subjects and CKD subjects in different stages were analyzed. The free Cys and free Cyss levels in their plasma were determined, and the reduction potential [Eh(Cyss/2Cys)] was calculated with the Nernst equation.

Results: Healthy plasma presented Eh(Cyss/2Cys) of -123 ± 7 mV. Plasma Eh(Cyss/2Cys) correlated significantly with creatinine levels (p < 0.0001, r = 0.62).

Conclusion: Plasma Eh(Cyss/2Cys) correlated with increased levels of plasma creatinine, supporting the view that uremia triggers oxidative stress. In addition, it may be used as a quantitative oxidative stress biomarker in uremic conditions.

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