Protective Effects of BDNF Overexpression Bone Marrow Stromal Cell Transplantation in Rat Models of Traumatic Brain Injury

Overview

Journal J Mol Neurosci

Specialties Molecular Biology
Neurology

Date 2012 Nov 13

PMID 23143881

Citations 18

Authors

Zhitao Wang

Weifeng Yao

Quanjun Deng

Xiaohui Zhang

Jianning Zhang

Affiliations

Soon will be listed here.

Abstract

Bone marrow stromal cells (MSCs) were used as cell therapy for various diseases in recent years. Some reports showed that transplanted MSCs promote functional recovery in animal models of brain trauma. But other studies indicate that tissue replacement by this method may not be the main source of therapeutic benefit. Neurotrophic factors such as brain-derived neurotrophic factor (BDNF) therapeutic potential may contribute to the recovery of function after trauma. Our previous study showed that BDNF-MSCs could promote the survival of neurons in neuronal injured models in vitro. The present study was undertaken to explore the therapeutic effects of MSCs transfected with BDNF in vivo. After intraventricular injection of MSCs-BDNF, BDNF levels were increased significantly in cerebrospinal fluid by ELISA. Further studies showed that treatment of traumatic brain injury with MSCs-BDNF could attenuate neuronal injury as measurement of biological behavior assessment. These studies demonstrate that by increasing the brain concentration of BDNF, intraventricularly transplanted MSCs-BDNF might play an important role in the treatment of traumatic brain injury and might be an optional therapeutic strategy.

Citing Articles

Effects of bone marrow mesenchymal stromal cells-derived therapies for experimental traumatic brain injury： A meta-analysis.

Chen C, Peng C, Hu Z, Ge L Heliyon. 2024; 10(3):e25050.

PMID: 38322864 PMC: 10844131. DOI: 10.1016/j.heliyon.2024.e25050.

Potential Neuroprotective Role of Neurotrophin in Traumatic Brain Injury.

Yap R, Kumar J, Teoh S CNS Neurol Disord Drug Targets. 2024; 23(10):1189-1202.

PMID: 38279761 DOI: 10.2174/0118715273289222231219094225.

Porous Silicon Nanoparticles Targeted to the Extracellular Matrix for Therapeutic Protein Delivery in Traumatic Brain Injury.

Waggoner L, Kang J, Zuidema J, Vijayakumar S, Hurtado A, Sailor M Bioconjug Chem. 2022; 33(9):1685-1697.

PMID: 36017941 PMC: 9492643. DOI: 10.1021/acs.bioconjchem.2c00305.

Stem Cell Transplantation Therapy and Neurological Disorders: Current Status and Future Perspectives.

Rahman M, Islam M, Islam M, Harun-Or-Rashid M, Islam M, Abdullah S Biology (Basel). 2022; 11(1).

PMID: 35053145 PMC: 8772847. DOI: 10.3390/biology11010147.

Systematic review and meta-analysis of preclinical studies testing mesenchymal stromal cells for traumatic brain injury.

Pischiutta F, Caruso E, Lugo A, Cavaleiro H, Stocchetti N, Citerio G NPJ Regen Med. 2021; 6(1):71.

PMID: 34716332 PMC: 8556393. DOI: 10.1038/s41536-021-00182-8.

References

Mu J, Li W, Yao Z, Zhou X . Deprivation of endogenous brain-derived neurotrophic factor results in impairment of spatial learning and memory in adult rats. Brain Res. 1999; 835(2):259-65. DOI: 10.1016/s0006-8993(99)01592-9. View

Bocker W, Yin Z, Drosse I, Haasters F, Rossmann O, Wierer M . Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer. J Cell Mol Med. 2008; 12(4):1347-59. PMC: 3865677. DOI: 10.1111/j.1582-4934.2008.00299.x. View

Assmus B, Schachinger V, Teupe C, Britten M, Lehmann R, Dobert N . Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI). Circulation. 2002; 106(24):3009-17. DOI: 10.1161/01.cir.0000043246.74879.cd. View

Kocher A, Schuster M, Szabolcs M, Takuma S, Burkhoff D, Wang J . Neovascularization of ischemic myocardium by human bone-marrow-derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function. Nat Med. 2001; 7(4):430-6. DOI: 10.1038/86498. View

Mezey E, Chandross K, Harta G, Maki R, McKercher S . Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow. Science. 2000; 290(5497):1779-82. DOI: 10.1126/science.290.5497.1779. View

Terada N, Hamazaki T, Oka M, Hoki M, Mastalerz D, Nakano Y . Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature. 2002; 416(6880):542-5. DOI: 10.1038/nature730. View

Kang H, Schuman E . A requirement for local protein synthesis in neurotrophin-induced hippocampal synaptic plasticity. Science. 1996; 273(5280):1402-6. DOI: 10.1126/science.273.5280.1402. View

Lipsky R, Marini A . Brain-derived neurotrophic factor in neuronal survival and behavior-related plasticity. Ann N Y Acad Sci. 2007; 1122:130-43. DOI: 10.1196/annals.1403.009. View

Clark B, Keating A . Biology of bone marrow stroma. Ann N Y Acad Sci. 1995; 770:70-8. DOI: 10.1111/j.1749-6632.1995.tb31044.x. View

10.

Diogenes M, Costenla A, Lopes L, Jeronimo-Santos A, Sousa V, Fontinha B . Enhancement of LTP in aged rats is dependent on endogenous BDNF. Neuropsychopharmacology. 2011; 36(9):1823-36. PMC: 3154100. DOI: 10.1038/npp.2011.64. View

11.

Mahmood A, Lu D, Wang L, Chopp M . Intracerebral transplantation of marrow stromal cells cultured with neurotrophic factors promotes functional recovery in adult rats subjected to traumatic brain injury. J Neurotrauma. 2003; 19(12):1609-17. DOI: 10.1089/089771502762300265. View

12.

Wang Z, Deng Q, Zhang X, Zhang J . Treatment of injured neurons with bone marrow stem cells cotransfected by hTERT and Ad-BDNF in vitro. J Mol Neurosci. 2009; 38(3):265-72. DOI: 10.1007/s12031-009-9208-5. View

13.

Philips M, Mattiasson G, Wieloch T, Bjorklund A, Johansson B, Tomasevic G . Neuroprotective and behavioral efficacy of nerve growth factor-transfected hippocampal progenitor cell transplants after experimental traumatic brain injury. J Neurosurg. 2001; 94(5):765-74. DOI: 10.3171/jns.2001.94.5.0765. View

14.

Li Y, Chopp M, Chen J, Wang L, Gautam S, Xu Y . Intrastriatal transplantation of bone marrow nonhematopoietic cells improves functional recovery after stroke in adult mice. J Cereb Blood Flow Metab. 2000; 20(9):1311-9. DOI: 10.1097/00004647-200009000-00006. View

15.

Bolton M, Pittman A, Lo D . Brain-derived neurotrophic factor differentially regulates excitatory and inhibitory synaptic transmission in hippocampal cultures. J Neurosci. 2000; 20(9):3221-32. PMC: 6773110. View

16.

Mahmood A, Lu D, Wang L, Li Y, Lu M, Chopp M . Treatment of traumatic brain injury in female rats with intravenous administration of bone marrow stromal cells. Neurosurgery. 2002; 49(5):1196-203; discussion 1203-4. View

17.

Cheng S, Yang J, Rifas L, Zhang S, Avioli L . Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrinology. 1994; 134(1):277-86. DOI: 10.1210/endo.134.1.8275945. View

18.

Chiaretti A, Piastra M, Polidori G, Di Rocco C, Caresta E, Antonelli A . Correlation between neurotrophic factor expression and outcome of children with severe traumatic brain injury. Intensive Care Med. 2003; 29(8):1329-38. DOI: 10.1007/s00134-003-1852-6. View

19.

Koc O, Gerson S, Cooper B, Dyhouse S, Haynesworth S, Caplan A . Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy. J Clin Oncol. 2000; 18(2):307-16. DOI: 10.1200/JCO.2000.18.2.307. View

20.

Beni-Adani L, Gozes I, Cohen Y, Assaf Y, Steingart R, Brenneman D . A peptide derived from activity-dependent neuroprotective protein (ADNP) ameliorates injury response in closed head injury in mice. J Pharmacol Exp Ther. 2000; 296(1):57-63. View