Functional Characterization of 14 Pht1 Family Genes in Yeast and Their Expressions in Response to Nutrient Starvation in Soybean

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Nov 8

PMID 23133521

Citations 40

Authors

Lu Qin

Yongxiang Guo

Liyu Chen

Ruikang Liang

Mian Gu

Guohua Xu

Jing Zhao

Thomas Walk

Hong Liao

Affiliations

Soon will be listed here.

Abstract

Background: Phosphorus (P) is essential for plant growth and development. Phosphate (Pi) transporter genes in the Pht1 family play important roles in Pi uptake and translocation in plants. Although Pht1 family genes have been well studied in model plants, little is known about their functions in soybean, an important legume crop worldwide.

Principal Findings: We identified and isolated a complete set of 14 Pi transporter genes (GmPT1-14) in the soybean genome and categorized them into two subfamilies based on phylogenetic analysis. Then, an experiment to elucidate Pi transport activity of the GmPTs was carried out using a yeast mutant defective in high-affinity Pi transport. Results showed that 12 of the 14 GmPTs were able to complement Pi uptake of the yeast mutant with Km values ranging from 25.7 to 116.3 µM, demonstrating that most of the GmPTs are high-affinity Pi transporters. Further results from qRT-PCR showed that the expressions of the 14 GmPTs differed not only in response to P availability in different tissues, but also to other nutrient stresses, including N, K and Fe deficiency, suggesting that besides functioning in Pi uptake and translocation, GmPTs might be involved in synergistic regulation of mineral nutrient homeostasis in soybean.

Conclusions: The comprehensive analysis of Pi transporter function in yeast and expression responses to nutrition starvation of Pht1 family genes in soybean revealed their involvement in other nutrient homeostasis besides P, which could help to better understand the regulation network among ion homeostasis in plants.

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References

Schachtman , Reid , Ayling . Phosphorus Uptake by Plants: From Soil to Cell. Plant Physiol. 1998; 116(2):447-53. PMC: 1539172. DOI: 10.1104/pp.116.2.447. View

Picault N, Hodges M, Palmieri L, Palmieri F . The growing family of mitochondrial carriers in Arabidopsis. Trends Plant Sci. 2004; 9(3):138-46. DOI: 10.1016/j.tplants.2004.01.007. View

Wang Y, Garvin D, Kochian L . Rapid induction of regulatory and transporter genes in response to phosphorus, potassium, and iron deficiencies in tomato roots. Evidence for cross talk and root/rhizosphere-mediated signals. Plant Physiol. 2002; 130(3):1361-70. PMC: 166655. DOI: 10.1104/pp.008854. View

Secco D, Baumann A, Poirier Y . Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons. Plant Physiol. 2010; 152(3):1693-704. PMC: 2832267. DOI: 10.1104/pp.109.149872. View

Javot H, Penmetsa R, Terzaghi N, Cook D, Harrison M . A Medicago truncatula phosphate transporter indispensable for the arbuscular mycorrhizal symbiosis. Proc Natl Acad Sci U S A. 2007; 104(5):1720-5. PMC: 1785290. DOI: 10.1073/pnas.0608136104. View

Nagy R, Karandashov V, Chague V, Kalinkevich K, Tamasloukht M, Xu G . The characterization of novel mycorrhiza-specific phosphate transporters from Lycopersicon esculentum and Solanum tuberosum uncovers functional redundancy in symbiotic phosphate transport in solanaceous species. Plant J. 2005; 42(2):236-50. DOI: 10.1111/j.1365-313X.2005.02364.x. View

Wu Z, Zhao J, Gao R, Hu G, Gai J, Xu G . Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max. PLoS One. 2011; 6(6):e19752. PMC: 3115949. DOI: 10.1371/journal.pone.0019752. View

Mudge S, Rae A, Diatloff E, Smith F . Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J. 2002; 31(3):341-53. DOI: 10.1046/j.1365-313x.2002.01356.x. View

Harrison M, Dewbre G, Liu J . A phosphate transporter from Medicago truncatula involved in the acquisition of phosphate released by arbuscular mycorrhizal fungi. Plant Cell. 2002; 14(10):2413-29. PMC: 151226. DOI: 10.1105/tpc.004861. View

10.

Karandashov V, Bucher M . Symbiotic phosphate transport in arbuscular mycorrhizas. Trends Plant Sci. 2005; 10(1):22-9. DOI: 10.1016/j.tplants.2004.12.003. View

11.

Woody J, Severin A, Bolon Y, Joseph B, Diers B, Farmer A . Gene expression patterns are correlated with genomic and genic structure in soybean. Genome. 2011; 54(1):10-8. DOI: 10.1139/G10-090. View

12.

Nishimura M, Harashima S, Oshima Y . The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol Cell Biol. 1991; 11(6):3229-38. PMC: 360175. DOI: 10.1128/mcb.11.6.3229-3238.1991. View

13.

Chen A, Hu J, Sun S, Xu G . Conservation and divergence of both phosphate- and mycorrhiza-regulated physiological responses and expression patterns of phosphate transporters in solanaceous species. New Phytol. 2007; 173(4):817-831. DOI: 10.1111/j.1469-8137.2006.01962.x. View

14.

Gierth M, Maser P, Schroeder J . The potassium transporter AtHAK5 functions in K(+) deprivation-induced high-affinity K(+) uptake and AKT1 K(+) channel contribution to K(+) uptake kinetics in Arabidopsis roots. Plant Physiol. 2005; 137(3):1105-14. PMC: 1065410. DOI: 10.1104/pp.104.057216. View

15.

Nacry P, Canivenc G, Muller B, Azmi A, Van Onckelen H, Rossignol M . A role for auxin redistribution in the responses of the root system architecture to phosphate starvation in Arabidopsis. Plant Physiol. 2005; 138(4):2061-74. PMC: 1183395. DOI: 10.1104/pp.105.060061. View

16.

Daram P, Brunner S, Persson B, Amrhein N, Bucher M . Functional analysis and cell-specific expression of a phosphate transporter from tomato. Planta. 1998; 206(2):225-33. DOI: 10.1007/s004250050394. View

17.

Liu F, Chang X, Ye Y, Xie W, Wu P, Lian X . Comprehensive sequence and whole-life-cycle expression profile analysis of the phosphate transporter gene family in rice. Mol Plant. 2011; 4(6):1105-22. DOI: 10.1093/mp/ssr058. View

18.

Hammond J, Bennett M, Bowen H, Broadley M, Eastwood D, May S . Changes in gene expression in Arabidopsis shoots during phosphate starvation and the potential for developing smart plants. Plant Physiol. 2003; 132(2):578-96. PMC: 166999. DOI: 10.1104/pp.103.020941. View

19.

Rausch C, Bucher M . Molecular mechanisms of phosphate transport in plants. Planta. 2002; 216(1):23-37. DOI: 10.1007/s00425-002-0921-3. View

20.

Misson J, Raghothama K, Jain A, Jouhet J, Block M, Bligny R . A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proc Natl Acad Sci U S A. 2005; 102(33):11934-9. PMC: 1188001. DOI: 10.1073/pnas.0505266102. View