Burkholderia Denitrificans Sp. Nov., Isolated from the Soil of Dokdo Island, Korea

Overview

Journal J Microbiol

Specialty Microbiology

Date 2012 Nov 6

PMID 23124756

Citations 3

Authors

Chang-Muk Lee

Hang-Yeon Weon

Sang-Hong Yoon

Soo-Jin Kim

Bon-Sung Koo

Soon-Wo Kwon

Affiliations

Soon will be listed here.

Abstract

A novel, Gram-negative, bacterial strain KIS30-44(T) was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15-30°C, pH 5-9, 0-3% NaCl, and 950 mM KNO(3). KIS30-44(T) reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44(T) was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44(T) exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44(T) were C(16:0), C(18:1) ω7c and summed feature 3 (iso-C(15:0) 2-OH and C(16:1) ω7c), and the strain contained half the amount of C(17:0) cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonomic data indicate that KIS30-44(T) represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44(T) =KACC 12733(T) =DSM 24336(T)) is proposed.

Citing Articles

Paraburkholderia dokdonella sp. nov., isolated from a plant from the genus Campanula.

Jung M, Kang M, Lee K, Lee E, Park S J Microbiol. 2018; 57(2):107-112.

PMID: 30456756 DOI: 10.1007/s12275-019-8500-5.

Confirmation of Two Undescribed Fungal Species from Dokdo of Korea Based on Current Classification System Using Multi Loci.

Lee H, Nguyen T, Mun H, Lee H, Kim C, Lee H Mycobiology. 2016; 43(4):392-401.

PMID: 26839498 PMC: 4731643. DOI: 10.5941/MYCO.2015.43.4.392.

Molecular signatures and phylogenomic analysis of the genus Burkholderia: proposal for division of this genus into the emended genus Burkholderia containing pathogenic organisms and a new genus Paraburkholderia gen. nov. harboring environmental....

Sawana A, Adeolu M, Gupta R Front Genet. 2015; 5:429.

PMID: 25566316 PMC: 4271702. DOI: 10.3389/fgene.2014.00429.

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