A Hepatitis C Virus NS5A Phosphorylation Site That Regulates RNA Replication

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2012 Nov 2

PMID 23115292

Citations 24

Authors

K L Lemay

J Treadaway

I Angulo

T L Tellinghuisen

Affiliations

Soon will be listed here.

Abstract

The hepatitis C virus NS5A protein is essential for RNA replication and virion assembly. NS5A is phosphorylated on multiple residues during infections, but these sites remain uncharacterized. Here we identify serine 222 of genotype 2a NS5A as a phosphorylation site that functions as a negative regulator of RNA replication. This site is a component of the hyperphosphorylated form of NS5A, which is in good agreement with previous observations that hyperphosphorylation negatively affects replication.

Citing Articles

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Sequential Phosphorylation of Hepatitis C Virus NS5A Protein Requires the ATP-Binding Domain of NS3 Helicase.

Yu C, Lin P, Chiang C, Jen S, Lai Y, Hsu S J Virol. 2022; 96(7):e0010722.

PMID: 35293767 PMC: 9006904. DOI: 10.1128/jvi.00107-22.

Phenotypic analysis of mutations at residue 146 provides insights into the relationship between NS5A hyperphosphorylation and hepatitis C virus genome replication.

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Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication.

Klinker S, Stindt S, Gremer L, Bode J, Gertzen C, Gohlke H J Biol Chem. 2019; 294(18):7388-7402.

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Characterization of a Threonine-Rich Cluster in Hepatitis C Virus Nonstructural Protein 5A and Its Contribution to Hyperphosphorylation.

Schenk C, Meyrath M, Warnken U, Schnolzer M, Mier W, Harak C J Virol. 2018; 92(24).

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