Article: Effect of spironolactone on acid and alkaline phosphatase in the testes of albino rat

The biological activity of the mineralocorticoid antagonist, spironolactone depend upon its metabolism. In this study the effect of diuretic drug compound spironolactone on the acid and alkaline phosphatase in the testes of albino rat. The drug spironolactone was administered orally daily for 7(th), 14(th) and 21(st) days at the dose of 100 mg/kg body weight. Among them, the side effects of drug are that the degradation of alkaline phosphatase in the testes; while acid phosphatase increased significantly may be due to 7a-thiospironolactone is a tissue metabolite of spironolactone and may contribute to the testicular enzymes, therapeutic actions and some other side effects. It is suggested that the decrease and increase of alkaline and acid phosphatase in the testes of albino rat are the due to the side effects of spironolactone drug compound.

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References

1.

King E, JEGATHEESAN K . A method for the determination of tartratelabile, prostatic acid phosphatase in serum. J Clin Pathol. 1959; 12(1):85-9. PMC: 479856. DOI: 10.1136/jcp.12.1.85. View

2.

Stripp B, Taylor A, Bartter F, GILLETTE J, Loriaux D, Easley R . Effect of spironolactone on sex hormones in man. J Clin Endocrinol Metab. 1975; 41(4):777-81. DOI: 10.1210/jcem-41-4-777. View

3.

Fujita S, Uesugi T, Ohta M, Kitani K . Effect of spironolactone on hepatic microsomal monooxygenase and azoreductase activities. Res Commun Chem Pathol Pharmacol. 1982; 36(1):87-103. View

4.

Kind P, King E . Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954; 7(4):322-6. PMC: 1023845. DOI: 10.1136/jcp.7.4.322. View

5.

JENKINS A, Lechene C, Howards S . Concentrations of seven elements in the intraluminal fluids of the rat seminiferous tubules, rate testis, and epididymis. Biol Reprod. 1980; 23(5):981-7. DOI: 10.1095/biolreprod23.5.981. View

6.

STEELMAN S, Brooks J, Morgan E, PATANELLI D . Anti-androgenic activity of spironolactone. Steroids. 1969; 14(4):449-50. DOI: 10.1016/s0039-128x(69)80007-3. View

7.

Menard R, Stripp B, GILLETTE J . Spironolactone and testicular cytochrome P-450: decreased testosterone formation in several species and changes in hepatic drug metabolism. Endocrinology. 1974; 94(6):1628-36. DOI: 10.1210/endo-94-6-1628. View

8.

Setchell B . Secretions of the testis and epididymis. J Reprod Fertil. 1974; 37(1):165-77. DOI: 10.1530/jrf.0.0370165. View

9.

Ochs H, Greenblatt D, BODEM G, Smith T . Spironolactone. Am Heart J. 1978; 96(3):389-400. DOI: 10.1016/0002-8703(78)90052-2. View

10.

JENKINS A, Lechene C, Howards S . The effect of spironolactone on the elemental composition of the intraluminal fluids of the seminiferous tubules, rete testis and epididymis of the rat. J Urol. 1983; 129(4):851-4. DOI: 10.1016/s0022-5347(17)52398-8. View

11.

Los L, Coddington A, Ramjit H, Colby H . Identification of spironolactone metabolites in plasma and target organs of guinea pigs. Drug Metab Dispos. 1993; 21(6):1086-90. View

Effect of Spironolactone on Acid and Alkaline Phosphatase in the Testes of Albino Rat