Structure and Transcription of a Human Gene for H1 RNA, the RNA Component of Human RNase P

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1990 Jan 11

PMID 2308839

Citations 87

Authors

M Baer

T W Nilsen

C Costigan

S Altman

Affiliations

Soon will be listed here.

Abstract

The gene coding for H1 RNA, the RNA component of human RNase P, has been isolated and characterized from a human genomic DNA library. The sequence corresponding to the mature H1 RNA is almost identical to that previously identified using H1 RNA and a cDNA clone corresponding to it. The nucleotide sequence of the genomic clone contains an array of potential transcriptional control elements, some characteristic of transcription by RNA polymerase III and some characteristic of RNA polymerase II, as is also the case for U6 and certain other small stable RNAs. The transcription in vitro of the genomic clone shows that the gene is functional and is transcribed by RNA polymerase III. Southern hybridization analysis indicates that there is very likely only one copy of the gene for H1 RNA in the human genome.

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References

Murphy S, Tripodi M, Melli M . A sequence upstream from the coding region is required for the transcription of the 7SK RNA genes. Nucleic Acids Res. 1986; 14(23):9243-60. PMC: 311956. DOI: 10.1093/nar/14.23.9243. View

Lobo S, Hernandez N . A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter. Cell. 1989; 58(1):55-67. DOI: 10.1016/0092-8674(89)90402-9. View

Vournakis J, Efstratiadis A, Kafatos F . Electrophoretic patterns of deadenylylated chorion and globin mRNAs. Proc Natl Acad Sci U S A. 1975; 72(8):2959-63. PMC: 432898. DOI: 10.1073/pnas.72.8.2959. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Weil P, Segall J, Harris B, Ng S, Roeder R . Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates. J Biol Chem. 1979; 254(13):6163-73. View

Bogenhagen D, Sakonju S, Brown D . A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region. Cell. 1980; 19(1):27-35. DOI: 10.1016/0092-8674(80)90385-2. View

Bogenhagen D, Brown D . Nucleotide sequences in Xenopus 5S DNA required for transcription termination. Cell. 1981; 24(1):261-70. DOI: 10.1016/0092-8674(81)90522-5. View

Dignam J, Lebovitz R, Roeder R . Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983; 11(5):1475-89. PMC: 325809. DOI: 10.1093/nar/11.5.1475. View

Ciliberto G, Raugei G, Costanzo F, Dente L, Cortese R . Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii. Cell. 1983; 32(3):725-33. DOI: 10.1016/0092-8674(83)90058-2. View

10.

Altman S . Aspects of biochemical catalysis. Cell. 1984; 36(2):237-9. DOI: 10.1016/0092-8674(84)90216-2. View

11.

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

12.

Ullu E, Weiner A . Upstream sequences modulate the internal promoter of the human 7SL RNA gene. Nature. 1985; 318(6044):371-4. DOI: 10.1038/318371a0. View

13.

Weiner A, Deininger P, Efstratiadis A . Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu Rev Biochem. 1986; 55:631-61. DOI: 10.1146/annurev.bi.55.070186.003215. View

14.

Kaslow D . A rapid biochemical method for purifying lambda DNA from phage lysates. Nucleic Acids Res. 1986; 14(16):6767. PMC: 311680. DOI: 10.1093/nar/14.16.6767. View

15.

Carbon P, Murgo S, Ebel J, Krol A, Tebb G, Mattaj L . A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II. Cell. 1987; 51(1):71-9. DOI: 10.1016/0092-8674(87)90011-0. View

16.

Murphy S, Di Liegro C, Melli M . The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter. Cell. 1987; 51(1):81-7. DOI: 10.1016/0092-8674(87)90012-2. View

17.

Weiner A, Maizels N . tRNA-like structures tag the 3' ends of genomic RNA molecules for replication: implications for the origin of protein synthesis. Proc Natl Acad Sci U S A. 1987; 84(21):7383-7. PMC: 299300. DOI: 10.1073/pnas.84.21.7383. View

18.

Ares Jr M, Chung J, Giglio L, Weiner A . Distinct factors with Sp1 and NF-A specificities bind to adjacent functional elements of the human U2 snRNA gene enhancer. Genes Dev. 1987; 1(8):808-17. DOI: 10.1101/gad.1.8.808. View

19.

Kunkel G, Pederson T . Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used. Genes Dev. 1988; 2(2):196-204. DOI: 10.1101/gad.2.2.196. View

20.

Das G, Henning D, Wright D, Reddy R . Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III. EMBO J. 1988; 7(2):503-12. PMC: 454347. DOI: 10.1002/j.1460-2075.1988.tb02838.x. View